Xp54, the Xenopus homologue of human RNA helicase p54, is an integral component of stored mRNP particles in oocytes

M Ladomery, E Wade, John Sommerville

Research output: Contribution to journalArticlepeer-review

128 Citations (Scopus)

Abstract

In investigating the composition of stored (maternal) mRNP particles in Xenopus oocytes, attention has focussed primarily on the phosphoproteins pp60/56, which are Y-box proteins involved in a general packaging of mRNA, We now identify a third, abundant, integral component of stored mRNP particles, Xp54, which belongs to the family of DEAD-box RNA helicases, Xp54 was first detected by its ability to photocrosslink ATP Subsequent sequence analysis identifies Xp54 as a member of a helicase subfamily which includes: human p54, encoded at a chromosomal breakpoint in the B-cell lymphoma cell line, RC-kappa 8;Drosophila ME31 B, encoded by a maternally-expressed gene, and Saccharomyces pombe Ste13, cloned by complementation of the sterility mutant stela Expression studies reveal that the gene encoding Xp54 is transcribed maximally at early oogenesis: no transcripts are detected in adult tissues, other than ovary, Using a monospecific antibody raised against native Xp54, its presence in mRNP particles is confirmed by immunoblotting fractions bound to oligo(dT)-cellulose and separated by rate sedimentation and buoyant density, On isolating Xp54 from mRNP particles, it is shown to possess an ATP-dependent RNA helicase activity, Possible functions of Xp54 are discussed in relation to the assembly and utilization of mRNP particles.

Original languageEnglish
Pages (from-to)965-973
Number of pages9
JournalNucleic Acids Research
Volume25
Issue number5
DOIs
Publication statusPublished - 1 Mar 1997

Keywords

  • MESSENGER-RIBONUCLEOPROTEIN PARTICLES
  • YEAST SCHIZOSACCHAROMYCES-POMBE
  • DEAD-BOX FAMILY
  • BINDING-PROTEINS
  • LAEVIS OOCYTES
  • PROTO-ONCOGENE
  • CELL-DIVISION
  • GENE
  • TRANSLATION
  • EXPRESSION

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