X-ray structural studies of the fungal laccase from Cerrena maxima

Andrey V. Lyashenko, Isabel Bento, Viatcheslav N. Zaitsev, Nadezhda E. Zhukhlistova, Yuliya N. Zhukova, Azat G. Gabdoulkhakov, Fkaterina Y. Morgunova, Wolfgang Voelter, Galina S. Kachalova, Elena V. Stepanova, Ga V. Koroleva, Victor S. Lamzin, Vladimir I. Tishkov, Christian Betzel, Peter F. Lindley, Al'bert M. Mikhailov

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

Laccases are members of the blue multicopper oxidase family. These enzymes oxidize substrate molecules by accepting electrons at a mononuclear copper centre and transferring them to a trinuclear centre. Dioxygen binds to the trinuclear centre and following the transfer of four electrons is reduced to two molecules of water. The X-ray structure of a laccase from Cerrena maxima has been elucidated at 1.9 angstrom resolution using synchrotron data and the molecular replacement technique. The final refinement coefficients are R-cryst = 16.8% and R-free = 23.0%, with root mean square deviations on bond lengths and bond angles of 0.015 angstrom and 1.51 degrees, respectively. The type 1 copper centre has an isoleucine residue at the axial position and the "resting" state of the trinuclear centre comprises a single oxygen (OH) moiety asymmetrically disposed between the two type 3 copper ions and a water molecule attached to the type 2 ion. Several carbohydrate binding sites have been identified and the glycan chains appear to promote the formation of well-ordered crystals. Two tyrosine residues near the protein surface have been found in a nitrated state.

Original languageEnglish
Pages (from-to)963-973
Number of pages11
JournalJournal of Biological Inorganic Chemistry
Volume11
DOIs
Publication statusPublished - Nov 2006

Keywords

  • laccase
  • trinuclear copper site
  • glycans
  • nitrated tyrosine residues
  • WHITE-ROT FUNGI
  • CRYSTAL-STRUCTURE
  • ANGSTROM RESOLUTION
  • COPRINUS-CINEREUS
  • CORIOLUS-HIRSUTUS
  • ELECTRON-TRANSFER
  • FULL COMPLEMENT
  • COPPER PROTEINS
  • NITRIC-OXIDE
  • REDUCTION

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