Wide-field multiphoton imaging with TRAFIX

Adrià Escobet-Montalbán; Philip Wijesinghe; Mingzhou Chen; Kishan Dholakia

doi:10.1117/12.2508373

Wide-field multiphoton imaging with TRAFIX

Adrià Escobet-Montalbán, Philip Wijesinghe, Mingzhou Chen, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Optical approaches have broadened their impact in recent years with innovations in both wide-field and super-resolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multiphoton images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while signifcantly reducing photo bleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.

Original language	English
Title of host publication	Multiphoton Microscopy in the Biomedical Sciences XIX
Editors	Ammasi Periasamy, Peter T. C. So, Karsten König
Publisher	Society of Photo-Optical Instrumentation Engineers
Pages	49
Number of pages	9
DOIs	https://doi.org/10.1117/12.2508373
Publication status	Published - 22 Feb 2019
Event	Multiphoton Microscopy in the Biomedical Sciences XIX - The Moscone Center, San Francisco, United States Duration: 2 Feb 2019 → 7 Feb 2019 https://spie.org/PWB/conferencedetails/multiphoton-microscopy?SSO=1

Publication series

Name	Proceedings of SPIE
Publisher	Society of Photo-optical Instrumentation Engineers
Volume	10882
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Multiphoton Microscopy in the Biomedical Sciences XIX
Abbreviated title	BIOS
Country/Territory	United States
City	San Francisco
Period	2/02/19 → 7/02/19
Internet address	https://spie.org/PWB/conferencedetails/multiphoton-microscopy?SSO=1

Keywords

Temporal focusing
Single-pixel imaging
Scattering media
Multiphoton microscopy
Three-photon compressive sensing

Access to Document

10.1117/12.2508373

Montalban-2019-Wide-field-BIOS-108821G
© 2019, SPIE. This work has been made available online in accordance with the publisher's policies. This is the final published version of the work, which was originally published at https://doi.org/10.1117/12.2508373
Final published version, 1.62 MB

Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world
Dholakia, K. (PI) & Gather, M. C. (CoI)
1/08/17 → 31/07/22
Project: Standard
H2020 MSCA ITN - BE-OPTICAL: H2020 Marie Curie ITN 2015 BE-OPTICAL
Dholakia, K. (PI)
European Commission
1/10/15 → 30/09/19
Project: Standard
Shaped light at the interface: Shaped Light at the Interface
Dholakia, K. (PI), Brown, C. T. A. (CoI), Di Falco, A. (CoI) & Mazilu, M. (CoI)
EPSRC
1/09/14 → 31/08/19
Project: Standard

Cite this

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title = "Wide-field multiphoton imaging with TRAFIX",

abstract = "Optical approaches have broadened their impact in recent years with innovations in both wide-field and super-resolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multiphoton images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while signifcantly reducing photo bleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.",

keywords = "Temporal focusing, Single-pixel imaging, Scattering media, Multiphoton microscopy, Three-photon compressive sensing",

author = "Adri{\`a} Escobet-Montalb{\'a}n and Philip Wijesinghe and Mingzhou Chen and Kishan Dholakia",

note = "This work is supported by the UK Engineering and Physical Sciences Research Council for funding through grants EP/P030017/1 and EP/M000869/1, and has received funding from the European Union's Horizon 2020 Programme through the project Advanced BiomEdical OPTICAL Imaging and Data Analysis (BE-OPTICAL) under grant agreement no. 675512. ; Multiphoton Microscopy in the Biomedical Sciences XIX, BIOS ; Conference date: 02-02-2019 Through 07-02-2019",

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doi = "10.1117/12.2508373",

language = "English",

series = "Proceedings of SPIE",

publisher = "Society of Photo-Optical Instrumentation Engineers",

pages = "49",

editor = "Ammasi Periasamy and So, {Peter T. C.} and Karsten K{\"o}nig",

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Escobet-Montalbán, A, Wijesinghe, P, Chen, M & Dholakia, K 2019, Wide-field multiphoton imaging with TRAFIX. in A Periasamy, PTC So & K König (eds), Multiphoton Microscopy in the Biomedical Sciences XIX., 10882G, Proceedings of SPIE, vol. 10882, Society of Photo-Optical Instrumentation Engineers, pp. 49, Multiphoton Microscopy in the Biomedical Sciences XIX, San Francisco, California, United States, 2/02/19. https://doi.org/10.1117/12.2508373

Wide-field multiphoton imaging with TRAFIX. / Escobet-Montalbán, Adrià; Wijesinghe, Philip; Chen, Mingzhou et al.
Multiphoton Microscopy in the Biomedical Sciences XIX. ed. / Ammasi Periasamy; Peter T. C. So; Karsten König. Society of Photo-Optical Instrumentation Engineers, 2019. p. 49 10882G (Proceedings of SPIE; Vol. 10882).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Wide-field multiphoton imaging with TRAFIX

AU - Escobet-Montalbán, Adrià

AU - Wijesinghe, Philip

AU - Chen, Mingzhou

AU - Dholakia, Kishan

N1 - This work is supported by the UK Engineering and Physical Sciences Research Council for funding through grants EP/P030017/1 and EP/M000869/1, and has received funding from the European Union's Horizon 2020 Programme through the project Advanced BiomEdical OPTICAL Imaging and Data Analysis (BE-OPTICAL) under grant agreement no. 675512.

PY - 2019/2/22

Y1 - 2019/2/22

N2 - Optical approaches have broadened their impact in recent years with innovations in both wide-field and super-resolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multiphoton images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while signifcantly reducing photo bleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.

AB - Optical approaches have broadened their impact in recent years with innovations in both wide-field and super-resolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multiphoton images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while signifcantly reducing photo bleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.

KW - Temporal focusing

KW - Single-pixel imaging

KW - Scattering media

KW - Multiphoton microscopy

KW - Three-photon compressive sensing

U2 - 10.1117/12.2508373

DO - 10.1117/12.2508373

M3 - Conference contribution

T3 - Proceedings of SPIE

SP - 49

BT - Multiphoton Microscopy in the Biomedical Sciences XIX

A2 - Periasamy, Ammasi

A2 - So, Peter T. C.

A2 - König, Karsten

PB - Society of Photo-Optical Instrumentation Engineers

T2 - Multiphoton Microscopy in the Biomedical Sciences XIX

Y2 - 2 February 2019 through 7 February 2019

ER -

Wide-field multiphoton imaging with TRAFIX

Abstract

Publication series

Conference

Keywords

Access to Document

Fingerprint

Projects

Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world

H2020 MSCA ITN - BE-OPTICAL: H2020 Marie Curie ITN 2015 BE-OPTICAL

Shaped light at the interface: Shaped Light at the Interface

Cite this