Wide-field multiphoton imaging with TRAFIX

Adrià Escobet-Montalbán, Philip Wijesinghe, Mingzhou Chen, Kishan Dholakia

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)
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Optical approaches have broadened their impact in recent years with innovations in both wide-field and super-resolution imaging, which now underpin biological and medical sciences. Whilst these advances have been remarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovative approach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multiphoton images of fluorescent microscopic samples deep through scattering media without correction. It has been shown that it can image through biological samples such as rat brain or human colon tissue up to a depth of seven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photon microscopy show that the former can yield a five-fold higher signal-to-background ratio while signifcantly reducing photo bleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX with three-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combined with compressive sensing for biomedicine.
Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIX
EditorsAmmasi Periasamy, Peter T. C. So, Karsten König
PublisherSociety of Photo-Optical Instrumentation Engineers
Number of pages9
Publication statusPublished - 22 Feb 2019
EventMultiphoton Microscopy in the Biomedical Sciences XIX - The Moscone Center, San Francisco, United States
Duration: 2 Feb 20197 Feb 2019

Publication series

NameProceedings of SPIE
PublisherSociety of Photo-optical Instrumentation Engineers
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceMultiphoton Microscopy in the Biomedical Sciences XIX
Abbreviated titleBIOS
Country/TerritoryUnited States
CitySan Francisco
Internet address


  • Temporal focusing
  • Single-pixel imaging
  • Scattering media
  • Multiphoton microscopy
  • Three-photon compressive sensing


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