Projects per year
Abstract
Optical approaches to fluorescent, spectroscopic, and morphological imaging have made exceptional advances in the last decade. Super-resolution imaging and wide-field multiphoton imaging are now underpinning major advances across the biomedical sciences. While the advances have been startling, the key unmet challenge to date in all forms of optical imaging is to penetrate deeper. A number of schemes implement aberration correction or the use of complex photonics to address this need. In contrast, we approach this challenge by implementing a scheme that requires no a priori information about the medium nor its properties. Exploiting temporal focusing and single-pixel detection in our innovative scheme, we obtain wide-field two-photon images through various turbid media including a scattering phantom and tissue reaching a depth of up to seven scattering mean free path lengths. Our results show that it competes favorably with standard point-scanning two-photon imaging, with up to a fivefold improvement in signal-to-background ratio while showing significantly lower photobleaching.
Original language | English |
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Article number | eaau1338 |
Pages (from-to) | 1-9 |
Number of pages | 9 |
Journal | Science Advances |
Volume | 4 |
Issue number | 10 |
DOIs | |
Publication status | Published - 12 Oct 2018 |
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Dive into the research topics of 'Wide-field multiphoton imaging through scattering media without correction'. Together they form a unique fingerprint.Projects
- 3 Finished
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Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world
Dholakia, K. (PI) & Gather, M. C. (CoI)
1/08/17 → 31/07/22
Project: Standard
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H2020 MSCA ITN - BE-OPTICAL: H2020 Marie Curie ITN 2015 BE-OPTICAL
Dholakia, K. (PI)
1/10/15 → 30/09/19
Project: Standard
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Shaped light at the interface: Shaped Light at the Interface
Dholakia, K. (PI), Brown, C. T. A. (CoI), Di Falco, A. (CoI) & Mazilu, M. (CoI)
1/09/14 → 31/08/19
Project: Standard