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into arbitrary geometries. We demonstrate the use of temporal focusing with holographic optical tweezers in order to achieve depth-resolved two-photon imaging of trapped objects arranged in arbitrary three dimensional geometries using a single objective. Trapping allows independent position control of multiple objects by holographic beam shaping. Temporal focusing of ultrashort pulses providesawide-field two-photon depth-selective activation of fluorescent samples. We demonstrate wide-field depth-resolved illumination of both trapped fluorescent beads and trapped HL60 cells in suspension with full 3D positioning control. These approaches are compatible with implementation through scattering media and can be beneficial for emergent studies in colloidal science and particularly optogenetics, offering targeted photoactivation over a wide area with µm depth control precision.
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- 2 Finished
1/06/12 → 31/05/17
Data underpinning - Wide-field 3D optical imaging using temporal focusing for holographically trapped microparticles
Spesyvtsev, R. (Creator), Rendall, H. A. (Creator) & Dholakia, K. (Creator), University of St Andrews, 2015