Wide-field 3D optical imaging using temporal focusing for holographically trapped microparticles

Roman Spesyvtsev, Helen Anne Rendall, Kishan Dholakia

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
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Abstract

A contemporary challenge across the natural sciences is the simultaneous optical imaging or stimulation of small numbers of cells or colloidal particles organised
into arbitrary geometries. We demonstrate the use of temporal focusing with holographic optical tweezers in order to achieve depth-resolved two-photon imaging of trapped objects arranged in arbitrary three dimensional geometries using a single objective. Trapping allows independent position control of multiple objects by holographic beam shaping. Temporal focusing of ultrashort pulses providesawide-field two-photon depth-selective activation of fluorescent samples. We demonstrate wide-field depth-resolved illumination of both trapped fluorescent beads and trapped HL60 cells in suspension with full 3D positioning control. These approaches are compatible with implementation through scattering media and can be beneficial for emergent studies in colloidal science and particularly optogenetics, offering targeted photoactivation over a wide area with µm depth control precision.
Original languageEnglish
Pages (from-to)4847-4850
JournalOptics Letters
Volume40
Issue number21
DOIs
Publication statusPublished - 16 Oct 2015

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