TY - JOUR
T1 - Whole genome sequencing for surveillance of antimicrobial resistance in Actinobacillus pleuropneumoniae
AU - Bossé, Janine T.
AU - Li, Yanwen
AU - Rogers, Jon
AU - Crespo, Roberto Fernandez
AU - Li, Yinghui
AU - Chaudhuri, Roy R.
AU - Holden, Matthew T. G.
AU - Maskell, Duncan J.
AU - Tucker, Alexander W.
AU - Wren, Brendan W.
AU - Rycroft, Andrew N.
AU - Langford, Paul R.
AU - BRaDP1T Consortium
N1 - This work was supported by a Longer and Larger (LoLa) grant from the Biotechnology and Biological Sciences Research Council (BBSRC grant numbers BB/G020744/1, BB/G019177/1, BB/G019274/1, and BB/G018553/1), the UK Department for Environment, Food and Rural Affairs, and Zoetis (formerly Pfizer Animal Health) awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology (BRaDP1T) consortium. MH was supported by the Wellcome Trust (grant number 098051). JR was funded from the former AHVLA’s Research and Development Internal Investment Fund (grant number RD0030c).
PY - 2017/3/6
Y1 - 2017/3/6
N2 - The aim of this study was to evaluate the correlation between antimicrobial resistance (AMR) profiles of 96 clinical isolates of Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen, and the identification of AMR genes in whole genome sequence (wgs) data. Susceptibility of the isolates to nine antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, sulfisoxazole, tetracycline, tilmicosin, trimethoprim, and tylosin) was determined by agar dilution susceptibility test. Except for the macrolides tested, elevated MICs were highly correlated to the presence of AMR genes identified in wgs data using ResFinder or BLASTn. Of the isolates tested, 57% were resistant to tetracycline [MIC ≥ 4 mg/L; 94.8% with either tet(B) or tet(H)]; 48% to sulfisoxazole (MIC ≥ 256 mg/L or DD = 6; 100% with sul2), 20% to ampicillin (MIC ≥ 4 mg/L; 100% with blaROB-1), 17% to trimethoprim (MIC ≥ 32 mg/L; 100% with dfrA14), and 6% to enrofloxacin (MIC ≥ 0.25 mg/L; 100% with GyrAS83F). Only 33% of the isolates did not have detectable AMR genes, and were sensitive by MICs for the antimicrobial agents tested. Although 23 isolates had MIC ≥ 32 mg/L for tylosin, all isolates had MIC ≥ 32 mg/L for tylosin, all isolates had MIC ≤ 16 mg/L for both
erythromycin and tilmicosin, and no macrolide resistance genes or known
point mutations were detected. Other than the GyrAS83F mutation, the AMR
genes detected were mapped to potential plasmids. In addition to
presence on plasmid(s), the tet(B) gene was also found chromosomally either as part of a 56 kb integrative conjugative element (ICEApl1) in 21, or as part of a Tn7
insertion in 15 isolates. Our results indicate that, with the exception
of macrolides, wgs data can be used to accurately predict resistance of
A. pleuropneumoniae to the tested antimicrobial agents and provides added value for routine surveillance.
AB - The aim of this study was to evaluate the correlation between antimicrobial resistance (AMR) profiles of 96 clinical isolates of Actinobacillus pleuropneumoniae, an important porcine respiratory pathogen, and the identification of AMR genes in whole genome sequence (wgs) data. Susceptibility of the isolates to nine antimicrobial agents (ampicillin, enrofloxacin, erythromycin, florfenicol, sulfisoxazole, tetracycline, tilmicosin, trimethoprim, and tylosin) was determined by agar dilution susceptibility test. Except for the macrolides tested, elevated MICs were highly correlated to the presence of AMR genes identified in wgs data using ResFinder or BLASTn. Of the isolates tested, 57% were resistant to tetracycline [MIC ≥ 4 mg/L; 94.8% with either tet(B) or tet(H)]; 48% to sulfisoxazole (MIC ≥ 256 mg/L or DD = 6; 100% with sul2), 20% to ampicillin (MIC ≥ 4 mg/L; 100% with blaROB-1), 17% to trimethoprim (MIC ≥ 32 mg/L; 100% with dfrA14), and 6% to enrofloxacin (MIC ≥ 0.25 mg/L; 100% with GyrAS83F). Only 33% of the isolates did not have detectable AMR genes, and were sensitive by MICs for the antimicrobial agents tested. Although 23 isolates had MIC ≥ 32 mg/L for tylosin, all isolates had MIC ≥ 32 mg/L for tylosin, all isolates had MIC ≤ 16 mg/L for both
erythromycin and tilmicosin, and no macrolide resistance genes or known
point mutations were detected. Other than the GyrAS83F mutation, the AMR
genes detected were mapped to potential plasmids. In addition to
presence on plasmid(s), the tet(B) gene was also found chromosomally either as part of a 56 kb integrative conjugative element (ICEApl1) in 21, or as part of a Tn7
insertion in 15 isolates. Our results indicate that, with the exception
of macrolides, wgs data can be used to accurately predict resistance of
A. pleuropneumoniae to the tested antimicrobial agents and provides added value for routine surveillance.
KW - Animal infections
KW - Antimicrobial resistance genes
KW - Integrative conjugative elements
KW - Plasmids
KW - Genomics
KW - Respiratory tract
KW - Pasteurellaceae
UR - http://journal.frontiersin.org/article/10.3389/fmicb.2017.00311/full#supplementary-material
U2 - 10.3389/fmicb.2017.00311
DO - 10.3389/fmicb.2017.00311
M3 - Article
SN - 1664-302X
VL - 8
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
M1 - 311
ER -