Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy

Birgit De Smet; Derek S Sarovich; Erin P Price; Mark Mayo; Vanessa Theobald; Chun Kham; Seiha Heng; Thong Phe; Matthew T G Holden; Julian Parkhill; Sharon J Peacock; Brian G Spratt; Jan Jacobs; Peter Vandamme; Bart J Currie

doi:10.1128/JCM.02574-14

Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy

Birgit De Smet, Derek S Sarovich, Erin P Price, Mark Mayo, Vanessa Theobald, Chun Kham, Seiha Heng, Thong Phe, Matthew T G Holden, Julian Parkhill, Sharon J Peacock, Brian G Spratt, Jan Jacobs, Peter Vandamme, Bart J Currie

Research output: Contribution to journal › Article › peer-review

Abstract

Burkholderia pseudomallei isolates with shared multilocus sequence types (STs) have not been isolated from different continents. We identified two STs shared between Australia and Cambodia. Whole-genome analysis revealed substantial diversity within STs, correctly identified the Asian or Australian origin, and confirmed that these shared STs were due to homoplasy.

Original language	English
Pages (from-to)	323-326
Journal	Journal of Clinical Microbiology
Volume	53
Issue number	1
Early online date	12 Nov 2014
DOIs	https://doi.org/10.1128/JCM.02574-14
Publication status	Published - Jan 2015

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10.1128/JCM.02574-14

J. Clin. Microbiol.-2015-De Smet-323-6
Copyright © 2015, De Smet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license (http://creativecommons.org/licenses/by/3.0/)
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Matthew Holden
- mtghst-andrews.acuk
- School of Medicine - Director of Impact, Professor
- Biomedical Sciences Research Complex
- St Andrews Bioinformatics Unit
- Infection and Global Health Division
Person: Academic

Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy (dataset)
De Smet, B. (Creator), Sarovich, D. S. (Creator), Price, E. P. (Creator), Mayo, M. (Creator), Theobald, V. (Creator), Kham, C. (Creator), Heng, S. (Creator), Phe, T. (Creator), Holden, M. T. G. (Creator), Parkhill, J. (Creator), Peacock, S. J. (Creator), Spratt, B. G. (Creator), Jacobs, J. (Creator), Vandamme, P. (Creator) & Currie, B. J. (Creator), National Center for Biotechnology Information, 25 Jun 2014
https://www.ncbi.nlm.nih.gov/sra/ERR539773 and 5 more links, https://www.ncbi.nlm.nih.gov/sra/ERR539807, https://www.ncbi.nlm.nih.gov/sra/ERR539841, https://www.ncbi.nlm.nih.gov/sra/ERR298339, https://www.ncbi.nlm.nih.gov/sra/ERR298360, https://www.ncbi.nlm.nih.gov/sra/ERR298343 (show fewer)
Dataset

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De Smet, B., Sarovich, D. S., Price, E. P., Mayo, M., Theobald, V., Kham, C., Heng, S., Phe, T., Holden, M. T. G., Parkhill, J., Peacock, S. J., Spratt, B. G., Jacobs, J., Vandamme, P., & Currie, B. J. (2015). Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy. Journal of Clinical Microbiology, 53(1), 323-326. https://doi.org/10.1128/JCM.02574-14

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title = "Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy",

abstract = "Burkholderia pseudomallei isolates with shared multilocus sequence types (STs) have not been isolated from different continents. We identified two STs shared between Australia and Cambodia. Whole-genome analysis revealed substantial diversity within STs, correctly identified the Asian or Australian origin, and confirmed that these shared STs were due to homoplasy.",

author = "{De Smet}, Birgit and Sarovich, {Derek S} and Price, {Erin P} and Mark Mayo and Vanessa Theobald and Chun Kham and Seiha Heng and Thong Phe and Holden, {Matthew T G} and Julian Parkhill and Peacock, {Sharon J} and Spratt, {Brian G} and Jan Jacobs and Peter Vandamme and Currie, {Bart J}",

note = "Whole-genome sequencing of the four isolates used in this study was supported by Wellcome Trust grant 098051, awarded to the Wellcome Trust Sanger Institute. This work was also supported by project grants from the Australian National Health and Medical Research Council and the Wellcome Trust. S.J.P. receives funding from the NIHR Cambridge Biomedical Research Centre. ",

year = "2015",

month = jan,

doi = "10.1128/JCM.02574-14",

language = "English",

volume = "53",

pages = "323--326",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

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De Smet, B, Sarovich, DS, Price, EP, Mayo, M, Theobald, V, Kham, C, Heng, S, Phe, T, Holden, MTG, Parkhill, J, Peacock, SJ, Spratt, BG, Jacobs, J, Vandamme, P & Currie, BJ 2015, 'Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy', Journal of Clinical Microbiology, vol. 53, no. 1, pp. 323-326. https://doi.org/10.1128/JCM.02574-14

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T1 - Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy

AU - De Smet, Birgit

AU - Sarovich, Derek S

AU - Price, Erin P

AU - Mayo, Mark

AU - Theobald, Vanessa

AU - Kham, Chun

AU - Heng, Seiha

AU - Phe, Thong

AU - Holden, Matthew T G

AU - Parkhill, Julian

AU - Peacock, Sharon J

AU - Spratt, Brian G

AU - Jacobs, Jan

AU - Vandamme, Peter

AU - Currie, Bart J

N1 - Whole-genome sequencing of the four isolates used in this study was supported by Wellcome Trust grant 098051, awarded to the Wellcome Trust Sanger Institute. This work was also supported by project grants from the Australian National Health and Medical Research Council and the Wellcome Trust. S.J.P. receives funding from the NIHR Cambridge Biomedical Research Centre.

PY - 2015/1

Y1 - 2015/1

N2 - Burkholderia pseudomallei isolates with shared multilocus sequence types (STs) have not been isolated from different continents. We identified two STs shared between Australia and Cambodia. Whole-genome analysis revealed substantial diversity within STs, correctly identified the Asian or Australian origin, and confirmed that these shared STs were due to homoplasy.

AB - Burkholderia pseudomallei isolates with shared multilocus sequence types (STs) have not been isolated from different continents. We identified two STs shared between Australia and Cambodia. Whole-genome analysis revealed substantial diversity within STs, correctly identified the Asian or Australian origin, and confirmed that these shared STs were due to homoplasy.

U2 - 10.1128/JCM.02574-14

DO - 10.1128/JCM.02574-14

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SN - 0095-1137

VL - 53

SP - 323

EP - 326

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 1

ER -

Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy

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Matthew Holden

Datasets

Whole-genome sequencing confirms that Burkholderia pseudomallei multilocus sequence types common to both Cambodia and Australia are due to homoplasy (dataset)

Cite this