Wavefront corrected light sheet microscopy in turbid media

Heather Isla Campbell Dalgarno; Tomas Cizmar; Tom Vettenburg; Jonathan Nylk; Frank J Gunn-Moore; Kishan Dholakia

doi:10.1063/1.4710527

Wavefront corrected light sheet microscopy in turbid media

Heather Isla Campbell Dalgarno, Tomas Cizmar, Tom Vettenburg, Jonathan Nylk, Frank J Gunn-Moore, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an “optimal” light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.

Original language	English
Article number	191108
Journal	Applied Physics Letters
Volume	100
Issue number	19
DOIs	https://doi.org/10.1063/1.4710527
Publication status	Published - May 2012

Keywords

Light sheet microscopy
Optical clearing
Bessel light modes
Background rejection
Wavefront correction
Gaussian beam
Bessel beam

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10.1063/1.4710527

AIP100
© 2012 American Institute of Physics. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the American Institute of Physics. The following article appeared in Applied Physics Letters, Vol 100, Issue 19, and may be found online at: http://scitation.aip.org/content/aip/journal/apl/100/19/10.1063/1.4710527
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title = "Wavefront corrected light sheet microscopy in turbid media",

abstract = "Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an “optimal” light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.",

keywords = "Light sheet microscopy , Optical clearing, Bessel light modes, Background rejection , Wavefront correction , Gaussian beam, Bessel beam",

author = "Dalgarno, {Heather Isla Campbell} and Tomas Cizmar and Tom Vettenburg and Jonathan Nylk and Gunn-Moore, {Frank J} and Kishan Dholakia",

note = "This project was supported by the UK Engineering and Physical Sciences Research Council.",

year = "2012",

month = may,

doi = "10.1063/1.4710527",

language = "English",

volume = "100",

journal = "Applied Physics Letters",

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T1 - Wavefront corrected light sheet microscopy in turbid media

AU - Dalgarno, Heather Isla Campbell

AU - Cizmar, Tomas

AU - Vettenburg, Tom

AU - Nylk, Jonathan

AU - Gunn-Moore, Frank J

AU - Dholakia, Kishan

N1 - This project was supported by the UK Engineering and Physical Sciences Research Council.

PY - 2012/5

Y1 - 2012/5

N2 - Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an “optimal” light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.

AB - Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an “optimal” light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.

KW - Light sheet microscopy

KW - Optical clearing

KW - Bessel light modes

KW - Background rejection

KW - Wavefront correction

KW - Gaussian beam

KW - Bessel beam

U2 - 10.1063/1.4710527

DO - 10.1063/1.4710527

M3 - Article

SN - 0003-6951

VL - 100

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 19

M1 - 191108

ER -

Wavefront corrected light sheet microscopy in turbid media

Abstract

Keywords

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