Wavefront corrected light sheet microscopy in turbid media

Heather Isla Campbell Dalgarno, Tomas Cizmar, Tom Vettenburg, Jonathan Nylk, Frank J Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)
3 Downloads (Pure)

Abstract

Light sheet microscopy is a powerful method for three-dimensional imaging of large biological specimens. However, its imaging ability is greatly diminished by sample scattering and aberrations. Optical clearing, Bessel light modes, and background rejection have been employed in attempts to circumvent these deleterious effects. We present an in situ wavefront correction that offers a major advance by creating an “optimal” light sheet within a turbid sample. Crucially, we show that no tissue clearing or specialized sample preparation is required, and clear improvements in image quality and depth resolution are demonstrated both in Gaussian and Bessel beam-based light sheet modalities.
Original languageEnglish
Article number191108
JournalApplied Physics Letters
Volume100
Issue number19
DOIs
Publication statusPublished - May 2012

Keywords

  • Light sheet microscopy
  • Optical clearing
  • Bessel light modes
  • Background rejection
  • Wavefront correction
  • Gaussian beam
  • Bessel beam

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