Visualizing the molecular mode of motion from a correlative analysis of localization microscopy datasets

Carmine Di Rienzo, Paolo Annibale*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The determination of the mode and rapidity of motion of individual molecules within a biological sample is becoming a more and more common analysis in biophysical investigations. Single molecule tracking (SMT) techniques allow reconstructing the trajectories of individual molecules within a movie, provided that the position from one frame to the other can be correctly linked. The outcomes, however, appear to depend on the specific method used, and most techniques display a limitation to capture fast modes of motion in a crowded environment. We demonstrate here that the limitations encountered by conventional SMT can be significantly overcome by employing alternative approaches based on image spatial-temporal correlations, enabling to visually extract quantitative insights on the ensemble mode of motion of fluorescently labeled biomolecules that would otherwise be inaccessible.

Original languageEnglish
Pages (from-to)4503-4506
Number of pages4
JournalOptics Letters
Volume41
Issue number19
DOIs
Publication statusPublished - 1 Oct 2016

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