Visualization of podocyte substructure with structured illumination microscopy (SIM): a new approach to nephrotic disease

James Pullman; Jonathan Nylk; Elaine Catherine Campbell; Frank J Gunn-Moore; Michael B Prystowsky; Kishan Dholakia

doi:10.1364/BOE.7.000302

Visualization of podocyte substructure with structured illumination microscopy (SIM): a new approach to nephrotic disease

James Pullman, Jonathan Nylk, Elaine Catherine Campbell, Frank J Gunn-Moore, Michael B Prystowsky, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perimeter of the podocyte foot processes and compared them with EM in both normal and nephrotic disease biopsies. SIM images of normal glomeruli revealed curvilinear patterns of podocin densely covering capillary walls similar to podocyte foot processes seen by EM. Podocin staining of all nephrotic disease biopsies were significantly different than normal, corresponding to and better visualizing effaced foot processes seen by EM. The findings support the first potential use of SIM in the diagnosis of nephrotic disease.

Original language	English
Pages (from-to)	302-311
Journal	Biomedical Optics Express
Volume	7
Issue number	2
Early online date	6 Jan 2016
DOIs	https://doi.org/10.1364/BOE.7.000302
Publication status	Published - 1 Feb 2016

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VisualizationOfPodocyteSubstructureWithSIM_Revised
Copyright 2016 OSA. This work is made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at: https://dx.doi.org/10.1364/BOE.7.000302. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited.
Accepted author manuscript, 825 KB

Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light
Dholakia, K. (PI), Krauss, T. F. (CoI) & Samuel, I. D. W. (CoI)
EPSRC
1/06/12 → 31/05/17
Project: Standard

Data underpining: Visualization of Podocyte Substructure with Structured Illumination Microscopy (SIM): A New Approach to Nephrotic Disease
Pullman, J. (Creator), Nylk, J. (Creator), Campbell, E. C. (Creator), Gunn-Moore, F. J. (Creator), Prystowsky, M. B. (Creator) & Dholakia, K. (Creator), University of St Andrews, 5 Jan 2016
DOI: 10.17630/b1c13755-9d80-44f0-bf14-fded4259d80d
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abstract = "A detailed microscopic analysis of renal podocyte substructure is essential to understand and diagnose nephrotic kidney disease. Currently only time consuming electron microscopy (EM) can resolve this substructure. We used structured illumination microscopy (SIM) to examine frozen sections of renal biopsies stained with an immunofluorescence marker for podocin, a protein localized to the perimeter of the podocyte foot processes and compared them with EM in both normal and nephrotic disease biopsies. SIM images of normal glomeruli revealed curvilinear patterns of podocin densely covering capillary walls similar to podocyte foot processes seen by EM. Podocin staining of all nephrotic disease biopsies were significantly different than normal, corresponding to and better visualizing effaced foot processes seen by EM. The findings support the first potential use of SIM in the diagnosis of nephrotic disease.",

author = "James Pullman and Jonathan Nylk and Campbell, {Elaine Catherine} and Gunn-Moore, {Frank J} and Prystowsky, {Michael B} and Kishan Dholakia",

note = "We thank the UK Engineering and Physical Sciences Research Council under grant EP/J0177X/1, the RS Macdonald Charitable Trust, the BRAINS 600th anniversary appeal, and Dr. Killick for funding. KD acknowledges the award of a Royal Society Leverhulme Trust Senior Fellowship. ",

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Visualization of podocyte substructure with structured illumination microscopy (SIM): a new approach to nephrotic disease

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Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light

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Data underpining: Visualization of Podocyte Substructure with Structured Illumination Microscopy (SIM): A New Approach to Nephrotic Disease

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