Abstract
A series of vectors is described which enables the episomal expression of proteins fused to different tag sequences in Schizosaccharomyces pombe. Proteins can be expressed with their amino termini fused to GFP/EGFP, three copies of the HA or Pk epitopes or a combined tag which contains two copies of the myc epitope and six histidine residues (MH). Fusion of the carboxyl terminus of a protein to a tag is possible with GFP/EGFP or Pk. Expression of the fusion proteins is controlled by the medium strength mutant version of the regulatable nmt1 promoter. (C) 1998 Elsevier Science B.V. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 59-68 |
| Number of pages | 10 |
| Journal | Gene |
| Volume | 221 |
| Issue number | 1 |
| Publication status | Published - 9 Oct 1998 |
Keywords
- GFP
- nmt1
- epitope tag
- fission yeast
- GREEN FLUORESCENT PROTEIN
- FISSION YEAST
- MONOCLONAL-ANTIBODIES
- SEPTUM FORMATION
- MUTANTS
- DIVISION
- HOMOLOG
- PRODUCT
- EPITOPE