Vectors for the expression of tagged proteins in Schizosaccharomyces pombe

RA Craven, DJ Griffiths, KS Sheldrick, Richard Edward Randall, IM Hagan, AM Carr

Research output: Contribution to journalArticlepeer-review

197 Citations (Scopus)

Abstract

A series of vectors is described which enables the episomal expression of proteins fused to different tag sequences in Schizosaccharomyces pombe. Proteins can be expressed with their amino termini fused to GFP/EGFP, three copies of the HA or Pk epitopes or a combined tag which contains two copies of the myc epitope and six histidine residues (MH). Fusion of the carboxyl terminus of a protein to a tag is possible with GFP/EGFP or Pk. Expression of the fusion proteins is controlled by the medium strength mutant version of the regulatable nmt1 promoter. (C) 1998 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)59-68
Number of pages10
JournalGene
Volume221
Issue number1
Publication statusPublished - 9 Oct 1998

Keywords

  • GFP
  • nmt1
  • epitope tag
  • fission yeast
  • GREEN FLUORESCENT PROTEIN
  • FISSION YEAST
  • MONOCLONAL-ANTIBODIES
  • SEPTUM FORMATION
  • MUTANTS
  • DIVISION
  • HOMOLOG
  • PRODUCT
  • EPITOPE

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