Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

Martin Denis Ryan, P de Felipe, V Martin, ML Cortes, other 1

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.

Original languageEnglish
Pages (from-to)198-208
Number of pages11
JournalGene Therapy
Volume6
Publication statusPublished - Feb 1999

Keywords

  • bicistronic retroviral vectors
  • FMDV 2A
  • pac
  • HSV1tk
  • gene therapy
  • DICISTRONIC TRANSCRIPTION UNITS
  • MAMMALIAN-CELLS
  • LEADER PROTEIN
  • EXPRESSION
  • CLEAVAGE
  • POLYPROTEIN

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