Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

Martin Denis Ryan; P de Felipe; V Martin; ML Cortes; other 1

Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

Martin Denis Ryan, P de Felipe, V Martin, ML Cortes, other 1

Research output: Contribution to journal › Article › peer-review

Abstract

We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.

Original language	English
Pages (from-to)	198-208
Number of pages	11
Journal	Gene Therapy
Volume	6
Publication status	Published - Feb 1999

Keywords

bicistronic retroviral vectors
FMDV 2A
pac
HSV1tk
gene therapy
DICISTRONIC TRANSCRIPTION UNITS
MAMMALIAN-CELLS
LEADER PROTEIN
EXPRESSION
CLEAVAGE
POLYPROTEIN

Cite this

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title = "Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy",

abstract = "We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.",

keywords = "bicistronic retroviral vectors, FMDV 2A, pac, HSV1tk, gene therapy, DICISTRONIC TRANSCRIPTION UNITS, MAMMALIAN-CELLS, LEADER PROTEIN, EXPRESSION, CLEAVAGE, POLYPROTEIN",

author = "Ryan, {Martin Denis} and {de Felipe}, P and V Martin and ML Cortes and other 1",

year = "1999",

month = feb,

language = "English",

volume = "6",

pages = "198--208",

journal = "Gene Therapy",

issn = "0969-7128",

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T1 - Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

AU - Ryan, Martin Denis

AU - de Felipe, P

AU - Martin, V

AU - Cortes, ML

AU - 1, other

PY - 1999/2

Y1 - 1999/2

N2 - We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.

AB - We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.

KW - bicistronic retroviral vectors

KW - FMDV 2A

KW - pac

KW - HSV1tk

KW - gene therapy

KW - DICISTRONIC TRANSCRIPTION UNITS

KW - MAMMALIAN-CELLS

KW - LEADER PROTEIN

KW - EXPRESSION

KW - CLEAVAGE

KW - POLYPROTEIN

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UR - http://www.stockton-press.co.uk/gt/index.html

M3 - Article

SN - 0969-7128

VL - 6

SP - 198

EP - 208

JO - Gene Therapy

JF - Gene Therapy

ER -

Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy

Abstract

Keywords

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