Projects per year
Abstract
The mbd cluster encodes the anaerobic degradation of 3-methylbenzoate in the β-proteobacterium Azoarcus sp. CIB. The specific transcriptional regulation circuit that controls the expression of the mbd genes was investigated. The PO, PB1, and P3R promoters responsible for the expression of the mbd genes, their cognate MbdR transcriptional repressor, as well as the MbdR operator regions (ATACN10GTAT) have been characterized. The three-dimensional structure of MbdR has been solved revealing a conformation similar to that of other TetR family transcriptional regulators. The first intermediate of the catabolic pathway, i.e. 3-methylbenzoyl-CoA, was shown to act as the inducer molecule. An additional MbdR-dependent promoter, PA, which contributes to the expression of the CoA ligase that activates 3-methylbenzoate to 3-methylbenzoyl-CoA, was shown to be necessary for an efficient induction of the mbd genes. Our results suggest that the mbd cluster recruited a regulatory system based on the MbdR regulator and its target promoters to evolve a distinct central catabolic pathway that is only expressed for the anaerobic degradation of aromatic compounds that generate 3-methylbenzoyl-CoA as the central metabolite. All these results highlight the importance of the regulatory systems in the evolution and adaptation of bacteria to the anaerobic degradation of aromatic compounds.
Original language | English |
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Pages (from-to) | 12165-12183 |
Journal | Journal of Biological Chemistry |
Volume | 290 |
Early online date | 20 Mar 2015 |
DOIs | |
Publication status | Published - 8 May 2015 |
Keywords
- Transcriptional regulation
- Azoarcus
- Anaerobic degradation
- 3-methylbenzoate
Fingerprint
Dive into the research topics of 'Unraveling the specific regulation of the mdb central pathway for the anaerobic degradation of 3-methylbenzoate'. Together they form a unique fingerprint.Projects
- 2 Finished
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Exploring the mechanism: Exploring the mechanism and scope of the enzymatic formation of five membered ring
Naismith, J. (PI), Botting, C. H. (CoI), Koehnke, J. A. J. G. (CoI) & Schwarz-Linek, U. (CoI)
1/10/13 → 30/09/17
Project: Standard
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Exploring the mechanism: Exploring the mechanism and scope of the enzymatic formation of five membered ring
Schwarz-Linek, U. (PI)
1/10/13 → 30/09/17
Project: Standard
Datasets
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Crystal structure of MbdR regulator from Azoarcus sp. CIB
Juárez, J. F. (Creator), Liu, H. (Creator), Zamarro, M. T. (Creator), McMahon, S. A. (Creator), Liu, H. (Creator), Naismith, J. H. (Creator), Eberlein, C. (Creator), Boll, M. (Creator), Carmona, M. (Creator) & Diaz, E. (Creator), Protein Data Bank (PDB), 24 Dec 2014
DOI: 10.2210/pdb4UDS/pdb, https://www.rcsb.org/structure/4uds
Dataset