Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C -glycoside synthase/PRPP complex

Sisi Gao; Ashish Radadiya; Wenbo Li; Huanting Liu; Wen Zhu; Valérie De Crécy-Lagard; Nigel G.J. Richards; James H. Naismith

doi:10.1039/d0cc02834g

Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C -glycoside synthase/PRPP complex

Sisi Gao, Ashish Radadiya, Wenbo Li, Huanting Liu, Wen Zhu, Valérie De Crécy-Lagard, Nigel G.J. Richards^*, James H. Naismith

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The enzyme ForT catalyzes C–C bond formation between 5′-phosphoribosyl-1′-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5′-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.

Original language	English
Pages (from-to)	7617-7620
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	55
Early online date	9 Jun 2020
DOIs	https://doi.org/10.1039/d0cc02834g
Publication status	Published - 14 Jul 2020

Access to Document

10.1039/d0cc02834gLicence: CC BY

Gao_2020_CC_Uncovering_CC
Copyright © 2020 The Author(s). Open Access Article. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Final published version, 2.66 MBLicence: CC BY

6YQQ ForT-PRPP complex
Gao, S. (Creator), Radadiya, A. (Creator), Li, W. (Creator), Liu, H. (Creator), Zhu, W. (Creator), De Crécy-Lagard, V. (Creator), Richards, N. G. J. (Creator) & Naismith, J. (Creator), Protein Data Bank (PDB), 20 May 2020
DOI: 10.2210/pdb6yqq/pdb
Dataset

Cite this

@article{8f9807d54b3b4f87b8b83ab2c3c4570a,

title = "Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C -glycoside synthase/PRPP complex",

abstract = "The enzyme ForT catalyzes C–C bond formation between 5′-phosphoribosyl-1′-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5′-phosphate by Streptomyces kaniharaensis. We report the 2.5 {\AA} resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.",

author = "Sisi Gao and Ashish Radadiya and Wenbo Li and Huanting Liu and Wen Zhu and {De Cr{\'e}cy-Lagard}, Val{\'e}rie and Richards, {Nigel G.J.} and Naismith, {James H.}",

note = "Authors thank the Diamond Light Source for beam time allocation and beam line staff for assistance with data collection. Funding for these studies was provided by BBSRC (BB/T006161/1 & BB/T006188/1 to J. H. N. & N. G. J. R., respectively), and the National Institutes of Health (R01 GM129793 to V. d. C.-L.)",

year = "2020",

month = jul,

day = "14",

doi = "10.1039/d0cc02834g",

language = "English",

volume = "56",

pages = "7617--7620",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "55",

}

TY - JOUR

T1 - Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis

T2 - crystal structure of a C -glycoside synthase/PRPP complex

AU - Gao, Sisi

AU - Radadiya, Ashish

AU - Li, Wenbo

AU - Liu, Huanting

AU - Zhu, Wen

AU - De Crécy-Lagard, Valérie

AU - Richards, Nigel G.J.

AU - Naismith, James H.

N1 - Authors thank the Diamond Light Source for beam time allocation and beam line staff for assistance with data collection. Funding for these studies was provided by BBSRC (BB/T006161/1 & BB/T006188/1 to J. H. N. & N. G. J. R., respectively), and the National Institutes of Health (R01 GM129793 to V. d. C.-L.)

PY - 2020/7/14

Y1 - 2020/7/14

N2 - The enzyme ForT catalyzes C–C bond formation between 5′-phosphoribosyl-1′-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5′-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.

AB - The enzyme ForT catalyzes C–C bond formation between 5′-phosphoribosyl-1′-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5′-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.

U2 - 10.1039/d0cc02834g

DO - 10.1039/d0cc02834g

M3 - Article

C2 - 32515440

AN - SCOPUS:85088012360

SN - 1359-7345

VL - 56

SP - 7617

EP - 7620

JO - Chemical Communications

JF - Chemical Communications

IS - 55

ER -

Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C -glycoside synthase/PRPP complex

Abstract

Access to Document

Fingerprint

Datasets

6YQQ ForT-PRPP complex

Cite this