Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C -glycoside synthase/PRPP complex

Sisi Gao, Ashish Radadiya, Wenbo Li, Huanting Liu, Wen Zhu, Valérie De Crécy-Lagard, Nigel G.J. Richards*, James H. Naismith

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)
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Abstract

The enzyme ForT catalyzes C–C bond formation between 5′-phosphoribosyl-1′-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5′-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.
Original languageEnglish
Pages (from-to)7617-7620
Number of pages4
JournalChemical Communications
Volume56
Issue number55
Early online date9 Jun 2020
DOIs
Publication statusPublished - 14 Jul 2020

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  • 6YQQ ForT-PRPP complex

    Gao, S. (Creator), Radadiya, A. (Creator), Li, W. (Creator), Liu, H. (Creator), Zhu, W. (Creator), De Crécy-Lagard, V. (Creator), Richards, N. G. J. (Creator) & Naismith, J. (Creator), Protein Data Bank (PDB), 20 May 2020

    Dataset

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