Ultrastructure of the segmental giant neuron of crayfish

W. J. Heitler*, J. L.S. Cobb, K. Fraser

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

The ultrastructure of the crayfish segmental giant (SG) neuron is described, and compared to other identified and unidentified crayfish neurons. The SG was specifically stained by intracellular injection of horseradish peroxidase and is divided into four regions of interest. 1. In the dorsal region, finger-like dendrites of the SG make contact with the throughconducting giant fibres (GF). These contacts are physiologically defined rectifying electrical synapses. They are characterized by the presence of 30-95 nm agranular vesicles in the presynaptic GFs, some postsynaptic density in the SG, and a narrowing of the intermembrane cleft to ∼ 5nm. There is little evidence for connecting cytoplasmic bridges. Unidentified neurons make chemical input with either round or elliptical vesicle types onto SG bottlenecks close to the electrical synapses. 2. Ventral to the GFs, dendritic profiles of the SG make three sorts of contact with unidentified neurons, (a) Regions of close membrane apposition (∼ 5nm) are presumed to be electrical output synapses, but there are no vesicles such as at the input synapses, and, again, little sign of connecting bridges. (b) Chemical input is received from unidentified presynaptic neurons containing either round or elliptical vesicles. These synapses are characterized by 30-75 nm presynaptic agranular vesicles, widened cleft (∼ 20 nm), granular cleft material and postsynaptic density. There is no sign of any presynaptic density. (c) Very occasional SG profiles containing vesicles and making output synapses to unidentified neurons occur. 3. In the lateral neuropil at the edge of the ganglion the SG gives rise to a small tuft of very fine dendrites. These are nearly all laden with vesicles and ramify in a complex region of neuropil containing many small profiles which are also vesicle-laden. 4. The SG axon diminishes in diameter as it progresses along its peripheral nerve root, and finally terminates at a blind ending near the base of the swimmerets. It is sheathed along its entire length, and there is no sign of vesicles within it. We conclude that the SG axon makes no peripheral output.

Original languageEnglish
Pages (from-to)921-941
Number of pages21
JournalJournal of Neurocytology
Volume14
Issue number6
DOIs
Publication statusPublished - Dec 1985

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