Two plant-viral movement proteins traffic in the endocytic recycling pathway

S Haupt, G H Cowan, A Ziegler, A G Roberts, K J Oparka, L Torrance

Research output: Contribution to journalArticlepeer-review

169 Citations (Scopus)

Abstract

Many plant viruses exploit a conserved group of proteins known as the triple gene block (TGB) for cell-to-cell movement. Here, we investigated the interaction of two TGB proteins (TGB2 and TGB3) of Potato mop-top virus (PMTV), with components of the secretory and endocytic pathways when expressed as N-terminal fusions to green fluorescent protein or monomeric red fluorescent protein (mRFP). Our studies revealed that fluorophore-labeled TGB2 and TGB3 showed an early association with the endoplasmic reticulum (ER) and colocalized in motile granules that used the ER-actin network for intracellular movement. Both proteins increased the size exclusion limit of plasmodesmata, and TGB3 accumulated at plasmodesmata in the absence of TGB2. TGB3 contains a putative Tyr-based sorting motif, mutations in which abolished ER localization and plasmodesmatal targeting. Later in the expression cycle, both fusion proteins were incorporated into vesicular structures. TGB2 associated with these structures on its own, but TGB3 could not be incorporated into the vesicles in the absence of TGB2. Moreover, in addition to localization to the ER and motile granules, mRFP-TGB3 was incorporated into vesicles when expressed in PMTV-infected epidermal cells, indicating recruitment by virus-expressed TGB2. The TGB fusion protein-containing vesicles were labeled with FM4-64, a marker for plasma membrane internalization and components of the endocytic pathway. TGB2 also colocalized in vesicles with Ara7, a Rab5 ortholog that marks the early endosome. Protein interaction analysis revealed that recombinant TGB2 interacted with a tobacco protein belonging to the highly conserved RME-8 family of J-domain chaperones, shown to be essential for endocytic trafficking in Caenorhabditis elegans and Drosophila melanogaster. Collectively, the data indicate the involvement of the endocytic pathway in viral intracellular movement, the implications of which are discussed.

Original languageEnglish
Pages (from-to)164-181
Number of pages18
JournalThe Plant Cell
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 2005

Keywords

  • CELL-TO-CELL
  • TRIPLE GENE BLOCK
  • TOBACCO-MOSAIC-VIRUS
  • J-DOMAIN PROTEIN
  • ENDOPLASMIC-RETICULUM
  • GOLGI-APPARATUS
  • CAENORHABDITIS-ELEGANS
  • COAT PROTEIN
  • POTATO
  • RNA

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