Two isoforms of the Na+/K+/2Cl- cotransporter (NKCC1) are expressed in the European eel (Anguilla anguilla).

Christopher Paul Cutler, Gordon Cramb

Research output: Contribution to journalArticlepeer-review

97 Citations (Scopus)

Abstract

Two cDNA isoforms of the NKCC1 secretory cotransporter have been isolated from the European eel. The NKCC1a isoform exhibited mRNA expression in a wide range of tissues in a similar fashion to marrimals, whereas NKCC1b was expressed primarily in the brain. The effect of freshwater (FW) to seawater (SW) transfer on NKCC1a expression was dependent on the developmental stage, In non-migratory yellow eels, NKCC1a mRNA expression in the gill was transiently up-regulated 4.3-fold after 2 days but also Subsequently by 2.5-6-fold 3 weeks after SW transfer. Gill NKCC1a expression was localised mainly in branchial chloride cells of SW acelimated yellow eels. In contrast to yellow eels, NKCC1a mRNA abundance was not significantly different following SW acclimation in silver eel gill NKCC1a mRNA abundance decreased in the kidney following SW acclimation and this may correlate with lower tubular ion fluid secretion and urine flow rates in SW teleosts. Kidney NKCC1a mRNA expression in silver eels was also significantly lower than in yellows eels, suggesting some pre-acclimation of mRNA levels. NKCC1a mRNA was expressed at similar low levels in the middle intestine of FW- and SW-acclimated yellow or silver eels, suggesting the presence of an ion secretory mechanism in this gilt segment. (C) 2002 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)92-103
Number of pages12
JournalBiochimica et Biophysica Acta
Volume1566
Publication statusPublished - 13 Nov 2002

Keywords

  • osmoregulation
  • teleost fish
  • CCC
  • NYCC2
  • NCC
  • KCC
  • BSCI
  • BSC2
  • K-CL COTRANSPORTER
  • FUNCTIONAL EXPRESSION
  • NA+-K+-2CL(-) COTRANSPORTER
  • FUNDULUS-HETEROCLITUS
  • CHLORIDE TRANSPORT
  • MOLECULAR-CLONING
  • CYCLASE-C
  • CELLS
  • SEQUENCE
  • LOCALIZATION

Fingerprint

Dive into the research topics of 'Two isoforms of the Na+/K+/2Cl- cotransporter (NKCC1) are expressed in the European eel (Anguilla anguilla).'. Together they form a unique fingerprint.

Cite this