Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation

Charlotte H. Hulme; Emma L. Wilson; Heidi R. Fuller; Sally Roberts; James B. Richardson; Pete Gallacher; Mandy J. Peffers; Sally L. Shirran; Catherine H. Botting; Karina T. Wright

doi:10.1186/s13075-018-1573-4

Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation

Charlotte H. Hulme
, Emma L. Wilson
, Heidi R. Fuller
, Sally Roberts
, James B. Richardson
, Pete Gallacher
, Mandy J. Peffers
, Sally L. Shirran
, Catherine H. Botting
, Karina T. Wright

Research output: Contribution to journal › Article › peer-review

Abstract

Autologous chondrocyte implantation (ACI) has a failure rate of approximately 20%, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternative or individualised therapies can be offered. We previously used label-free quantitation (LF) with a dynamic range compression proteomic approach to assess the synovial fluid (SF) of ACI responders and non-responders. However, we were able to identify only a few differentially abundant proteins at baseline. In the present study, we built upon these previous findings by assessing higher-abundance proteins within this SF, providing a more global proteomic analysis on the basis of which more of the biology underlying ACI success or failure can be understood.

Original language	English
Article number	87
Number of pages	17
Journal	Arthritis Research & Therapy
Volume	20
DOIs	https://doi.org/10.1186/s13075-018-1573-4
Publication status	Published - 2 May 2018

Keywords

Autologous chondrocyte implantation (ACI)
iTRAQ proteomics
Label-free quantitation proteomics
Synovial fluid
Cartilage repair
Complement C1S subcomponent
Matrix metalloproteinase 3
MMP3

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© The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Final published version, 1.04 MBLicence: CC BY

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MaXis ESI QTOF mass spectrometer: Equipment only grant-Mass spectrometers for Proteomics, Lipidomics and focused Metabolomics research
Botting, C. (PI), Elliott, R. (CoI), Randall, R. (CoI), Smith, T. K. (CoI) & White, M. (CoI)
The Wellcome Trust
1/08/11 → 31/07/14
Project: Standard

Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation (dataset)
Hulme, C. H. (Creator), Wilson, E. L. (Creator), Fuller, H. R. (Creator), Roberts, S. (Creator), Richardson, J. B. (Creator), Gallacher, P. (Creator), Peffers, M. J. (Creator), Shirran, S. L. (Creator) & Botting, C. H. (Creator), Proteome Exchange, 3 May 2018
http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD008321
Dataset

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Hulme, C. H., Wilson, E. L., Fuller, H. R., Roberts, S., Richardson, J. B., Gallacher, P., Peffers, M. J., Shirran, S. L., Botting, C. H., & Wright, K. T. (2018). Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation. Arthritis Research & Therapy, 20, Article 87. https://doi.org/10.1186/s13075-018-1573-4

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title = "Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation",

abstract = "Autologous chondrocyte implantation (ACI) has a failure rate of approximately 20\%, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternative or individualised therapies can be offered. We previously used label-free quantitation (LF) with a dynamic range compression proteomic approach to assess the synovial fluid (SF) of ACI responders and non-responders. However, we were able to identify only a few differentially abundant proteins at baseline. In the present study, we built upon these previous findings by assessing higher-abundance proteins within this SF, providing a more global proteomic analysis on the basis of which more of the biology underlying ACI success or failure can be understood.",

keywords = "Autologous chondrocyte implantation (ACI), iTRAQ proteomics, Label-free quantitation proteomics, Synovial fluid, Cartilage repair, Complement C1S subcomponent, Matrix metalloproteinase 3, MMP3",

author = "Hulme, \{Charlotte H.\} and Wilson, \{Emma L.\} and Fuller, \{Heidi R.\} and Sally Roberts and Richardson, \{James B.\} and Pete Gallacher and Peffers, \{Mandy J.\} and Shirran, \{Sally L.\} and Botting, \{Catherine H.\} and Wright, \{Karina T.\}",

note = "We thank Arthritis Research UK for supporting this work via grants 19429, 20815 and 21122. MJP is supported through a Wellcome Trust Clinical Intermediate Fellowship. This work was supported by Wellcome Trust grant 094476/Z/10/Z, which funded the purchase of the TripleTOF 5600 mass spectrometer at the Biomedical Sciences Research Complex Mass Spectrometry and Proteomics Facility, University of St. Andrews (Fife, UK).",

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doi = "10.1186/s13075-018-1573-4",

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Hulme, CH, Wilson, EL, Fuller, HR, Roberts, S, Richardson, JB, Gallacher, P, Peffers, MJ, Shirran, SL, Botting, CH & Wright, KT 2018, 'Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation', Arthritis Research & Therapy, vol. 20, 87. https://doi.org/10.1186/s13075-018-1573-4

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AU - Hulme, Charlotte H.

AU - Wilson, Emma L.

AU - Fuller, Heidi R.

AU - Roberts, Sally

AU - Richardson, James B.

AU - Gallacher, Pete

AU - Peffers, Mandy J.

AU - Shirran, Sally L.

AU - Botting, Catherine H.

AU - Wright, Karina T.

N1 - We thank Arthritis Research UK for supporting this work via grants 19429, 20815 and 21122. MJP is supported through a Wellcome Trust Clinical Intermediate Fellowship. This work was supported by Wellcome Trust grant 094476/Z/10/Z, which funded the purchase of the TripleTOF 5600 mass spectrometer at the Biomedical Sciences Research Complex Mass Spectrometry and Proteomics Facility, University of St. Andrews (Fife, UK).

PY - 2018/5/2

Y1 - 2018/5/2

N2 - Autologous chondrocyte implantation (ACI) has a failure rate of approximately 20%, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternative or individualised therapies can be offered. We previously used label-free quantitation (LF) with a dynamic range compression proteomic approach to assess the synovial fluid (SF) of ACI responders and non-responders. However, we were able to identify only a few differentially abundant proteins at baseline. In the present study, we built upon these previous findings by assessing higher-abundance proteins within this SF, providing a more global proteomic analysis on the basis of which more of the biology underlying ACI success or failure can be understood.

AB - Autologous chondrocyte implantation (ACI) has a failure rate of approximately 20%, but it is yet to be fully understood why. Biomarkers are needed that can pre-operatively predict in which patients it is likely to fail, so that alternative or individualised therapies can be offered. We previously used label-free quantitation (LF) with a dynamic range compression proteomic approach to assess the synovial fluid (SF) of ACI responders and non-responders. However, we were able to identify only a few differentially abundant proteins at baseline. In the present study, we built upon these previous findings by assessing higher-abundance proteins within this SF, providing a more global proteomic analysis on the basis of which more of the biology underlying ACI success or failure can be understood.

KW - Autologous chondrocyte implantation (ACI)

KW - iTRAQ proteomics

KW - Label-free quantitation proteomics

KW - Synovial fluid

KW - Cartilage repair

KW - Complement C1S subcomponent

KW - Matrix metalloproteinase 3

KW - MMP3

U2 - 10.1186/s13075-018-1573-4

DO - 10.1186/s13075-018-1573-4

M3 - Article

SN - 1478-6362

VL - 20

JO - Arthritis Research & Therapy

JF - Arthritis Research & Therapy

M1 - 87

ER -

Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation

Abstract

Keywords

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Projects

MaXis ESI QTOF mass spectrometer: Equipment only grant-Mass spectrometers for Proteomics, Lipidomics and focused Metabolomics research

Datasets

Two independent proteomic approaches provide a comprehensive analysis of the synovial fluid proteome response to Autologous Chondrocyte Implantation (dataset)

Cite this