Two Holliday junction resolving enzymes in Sulfolobus solfataricus

M Kvaratskhelia, Malcolm Frederick White

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Holliday junction resolving enzymes bind specifically to four-way DNA junctions created by the process of homologous recombination, cleaving them to yield recombinant duplex DNA products. Homologous recombination is known to occur in the third domain of life, the archaea, and may constitute a simplified model for the corresponding eucaryal pathway, but has not been well characterised. Identification of a gene encoding an archaeal Holliday junction resolving enzyme, Hjc, has recently been reported in the euryarchaea, and an activity has been observed in the hyperthermophilic crenarchaeote Sulfolobus solfataricus. Here we report the identification, heterologous expression and characterisation of the Hjc protein from Sulfolobus. We demonstrate that Sulfolobus has two distinct junction resolving enzymes, Hjc and Hje, with differing substrate specificities. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)923-932
Number of pages10
JournalJournal of Molecular Biology
Volume297
Issue number4
Publication statusPublished - 7 Apr 2000

Keywords

  • Holliday junction
  • archaea
  • endonuclease
  • resolving enzyme
  • homologous recombination
  • SACCHAROMYCES-CEREVISIAE
  • ESCHERICHIA-COLI
  • RUVC RESOLVASE
  • DNA
  • RECOMBINATION
  • ENDONUCLEASE
  • RESOLUTION
  • PROTEIN
  • CCE1

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