Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase

R Sànchez-Fernàndez; S E Unkles; E I Campbell; J A Macro; E Cerdà-Olmedo; J R Kinghorn

doi:10.1007/BF00269853

Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase

R Sànchez-Fernàndez, S E Unkles, E I Campbell, J A Macro, E Cerdà-Olmedo, J R Kinghorn

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Abstract

A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.

Original language	English
Pages (from-to)	231-233
Number of pages	3
Journal	Molecular and General Genetics
Volume	225
Issue number	2
DOIs	https://doi.org/10.1007/BF00269853
Publication status	Published - Feb 1991

Keywords

Aspergillus niger
Blotting, Southern
Gibberella
Mutagenesis
Nitrate Reductase
Nitrate Reductases
Restriction Mapping
Transformation, Genetic

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title = "Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase",

abstract = "A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.",

keywords = "Aspergillus niger, Blotting, Southern, Gibberella, Mutagenesis, Nitrate Reductase, Nitrate Reductases, Restriction Mapping, Transformation, Genetic",

author = "R S{\`a}nchez-Fern{\`a}ndez and Unkles, \{S E\} and Campbell, \{E I\} and Macro, \{J A\} and E Cerd{\`a}-Olmedo and Kinghorn, \{J R\}",

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doi = "10.1007/BF00269853",

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T1 - Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase

AU - Sànchez-Fernàndez, R

AU - Unkles, S E

AU - Campbell, E I

AU - Macro, J A

AU - Cerdà-Olmedo, E

AU - Kinghorn, J R

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N2 - A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.

AB - A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.

KW - Aspergillus niger

KW - Blotting, Southern

KW - Gibberella

KW - Mutagenesis

KW - Nitrate Reductase

KW - Nitrate Reductases

KW - Restriction Mapping

KW - Transformation, Genetic

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U2 - 10.1007/BF00269853

DO - 10.1007/BF00269853

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C2 - 2005864

SN - 0026-8925

VL - 225

SP - 231

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JO - Molecular and General Genetics

JF - Molecular and General Genetics

IS - 2

ER -

Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase

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Keywords

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