Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase

R Sànchez-Fernàndez, S E Unkles, E I Campbell, J A Macro, E Cerdà-Olmedo, J R Kinghorn

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20 Citations (Scopus)

Abstract

A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.
Original languageEnglish
Pages (from-to)231-233
Number of pages3
JournalMolecular and General Genetics
Volume225
Issue number2
DOIs
Publication statusPublished - Feb 1991

Keywords

  • Aspergillus niger
  • Blotting, Southern
  • Gibberella
  • Mutagenesis
  • Nitrate Reductase
  • Nitrate Reductases
  • Restriction Mapping
  • Transformation, Genetic

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