Transformation of Gibberella fujikuroi: effect of the Aspergillus nidulans AMA1 sequence on frequency and integration

B Brückner; S E Unkles; K Weltring; J R Kinghorn

Transformation of Gibberella fujikuroi: effect of the Aspergillus nidulans AMA1 sequence on frequency and integration

B Brückner, S E Unkles, K Weltring, J R Kinghorn

Research output: Contribution to journal › Article › peer-review

Abstract

A stable and reproducible transformation selection system for Gibberella fujikuroi protoplasts based on the Aspergillus nidulans arg B gene, encoding ornithine transcarbamylase, has been developed. Inclusion into the vector of the A. nidulans DNA fragment (AMA1), which permits plasmid autonomous replication in A. nidulans, A. niger and A. oryzae, appeared to permit autonomous replication of G. fujikuroi although the transformation frequency was increased by only two-fold. Transformation was also achieved using the bacterial hygromycin B resistance gene under the control of G. pulicaris and A. nidulans promoters.

Original language	English
Pages (from-to)	313-6
Number of pages	4
Journal	Current Genetics
Volume	22
Issue number	4
Publication status	Published - Oct 1992

Keywords

Arginine
Aspergillus nidulans
Blotting, Southern
Cinnamates
DNA Transposable Elements
DNA, Fungal
Drug Resistance
Genetic Techniques
Gibberella
Hygromycin B
Mitosis
Mutagenesis
Nucleic Acid Hybridization
Plasmids
Transformation, Genetic

Cite this

@article{365bd3376a6a4068b5b7b7235c209d70,

title = "Transformation of Gibberella fujikuroi: effect of the Aspergillus nidulans AMA1 sequence on frequency and integration",

abstract = "A stable and reproducible transformation selection system for Gibberella fujikuroi protoplasts based on the Aspergillus nidulans arg B gene, encoding ornithine transcarbamylase, has been developed. Inclusion into the vector of the A. nidulans DNA fragment (AMA1), which permits plasmid autonomous replication in A. nidulans, A. niger and A. oryzae, appeared to permit autonomous replication of G. fujikuroi although the transformation frequency was increased by only two-fold. Transformation was also achieved using the bacterial hygromycin B resistance gene under the control of G. pulicaris and A. nidulans promoters.",

keywords = "Arginine, Aspergillus nidulans, Blotting, Southern, Cinnamates, DNA Transposable Elements, DNA, Fungal, Drug Resistance, Genetic Techniques, Gibberella, Hygromycin B, Mitosis, Mutagenesis, Nucleic Acid Hybridization, Plasmids, Transformation, Genetic",

author = "B Br{\"u}ckner and Unkles, {S E} and K Weltring and Kinghorn, {J R}",

year = "1992",

month = oct,

language = "English",

volume = "22",

pages = "313--6",

journal = "Current Genetics",

issn = "0172-8083",

publisher = "Springer-Verlag",

number = "4",

}

TY - JOUR

T1 - Transformation of Gibberella fujikuroi

T2 - effect of the Aspergillus nidulans AMA1 sequence on frequency and integration

AU - Brückner, B

AU - Unkles, S E

AU - Weltring, K

AU - Kinghorn, J R

PY - 1992/10

Y1 - 1992/10

N2 - A stable and reproducible transformation selection system for Gibberella fujikuroi protoplasts based on the Aspergillus nidulans arg B gene, encoding ornithine transcarbamylase, has been developed. Inclusion into the vector of the A. nidulans DNA fragment (AMA1), which permits plasmid autonomous replication in A. nidulans, A. niger and A. oryzae, appeared to permit autonomous replication of G. fujikuroi although the transformation frequency was increased by only two-fold. Transformation was also achieved using the bacterial hygromycin B resistance gene under the control of G. pulicaris and A. nidulans promoters.

AB - A stable and reproducible transformation selection system for Gibberella fujikuroi protoplasts based on the Aspergillus nidulans arg B gene, encoding ornithine transcarbamylase, has been developed. Inclusion into the vector of the A. nidulans DNA fragment (AMA1), which permits plasmid autonomous replication in A. nidulans, A. niger and A. oryzae, appeared to permit autonomous replication of G. fujikuroi although the transformation frequency was increased by only two-fold. Transformation was also achieved using the bacterial hygromycin B resistance gene under the control of G. pulicaris and A. nidulans promoters.

KW - Arginine

KW - Aspergillus nidulans

KW - Blotting, Southern

KW - Cinnamates

KW - DNA Transposable Elements

KW - DNA, Fungal

KW - Drug Resistance

KW - Genetic Techniques

KW - Gibberella

KW - Hygromycin B

KW - Mitosis

KW - Mutagenesis

KW - Nucleic Acid Hybridization

KW - Plasmids

KW - Transformation, Genetic

M3 - Article

C2 - 1327547

SN - 0172-8083

VL - 22

SP - 313

EP - 316

JO - Current Genetics

JF - Current Genetics

IS - 4

ER -

Transformation of Gibberella fujikuroi: effect of the Aspergillus nidulans AMA1 sequence on frequency and integration

Abstract

Keywords

Fingerprint

Cite this