Transformation of Aspergillus oryzae using the A. niger pyrG gene

I E Mattern; S Unkles; J R Kinghorn; P H Pouwels; C A van den Hondel

doi:10.1007/BF00327197

Transformation of Aspergillus oryzae using the A. niger pyrG gene

I E Mattern, S Unkles, J R Kinghorn, P H Pouwels, C A van den Hondel

Research output: Contribution to journal › Article › peer-review

Abstract

A transformation system for Aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per microgram of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analysis showed that vector DNA sequences were integrated into the chromosomal DNA, in various copy numbers and presumably at different sites. Efficient cotransformation of an unselectable gene was also shown. Under the conditions used no transformants were obtained with the equivalent pyr4 gene of Neurospora crassa.

Original language	English
Pages (from-to)	460-461
Number of pages	2
Journal	Molecular and General Genetics
Volume	210
Issue number	3
DOIs	https://doi.org/10.1007/BF00327197
Publication status	Published - Dec 1987

Keywords

Aspergillus
Aspergillus niger
Aspergillus oryzae
Carboxy-Lyases
Genetic Vectors
Orotidine-5'-Phosphate Decarboxylase
Selection, Genetic
Transformation, Genetic

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title = "Transformation of Aspergillus oryzae using the A. niger pyrG gene",

abstract = "A transformation system for Aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per microgram of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analysis showed that vector DNA sequences were integrated into the chromosomal DNA, in various copy numbers and presumably at different sites. Efficient cotransformation of an unselectable gene was also shown. Under the conditions used no transformants were obtained with the equivalent pyr4 gene of Neurospora crassa.",

keywords = "Aspergillus, Aspergillus niger, Aspergillus oryzae, Carboxy-Lyases, Genetic Vectors, Orotidine-5'-Phosphate Decarboxylase, Selection, Genetic, Transformation, Genetic",

author = "Mattern, {I E} and S Unkles and Kinghorn, {J R} and Pouwels, {P H} and {van den Hondel}, {C A}",

year = "1987",

month = dec,

doi = "10.1007/BF00327197",

language = "English",

volume = "210",

pages = "460--461",

journal = "Molecular and General Genetics",

issn = "0026-8925",

publisher = "Springer-Verlag",

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T1 - Transformation of Aspergillus oryzae using the A. niger pyrG gene

AU - Mattern, I E

AU - Unkles, S

AU - Kinghorn, J R

AU - Pouwels, P H

AU - van den Hondel, C A

PY - 1987/12

Y1 - 1987/12

N2 - A transformation system for Aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per microgram of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analysis showed that vector DNA sequences were integrated into the chromosomal DNA, in various copy numbers and presumably at different sites. Efficient cotransformation of an unselectable gene was also shown. Under the conditions used no transformants were obtained with the equivalent pyr4 gene of Neurospora crassa.

AB - A transformation system for Aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per microgram of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analysis showed that vector DNA sequences were integrated into the chromosomal DNA, in various copy numbers and presumably at different sites. Efficient cotransformation of an unselectable gene was also shown. Under the conditions used no transformants were obtained with the equivalent pyr4 gene of Neurospora crassa.

KW - Aspergillus

KW - Aspergillus niger

KW - Aspergillus oryzae

KW - Carboxy-Lyases

KW - Genetic Vectors

KW - Orotidine-5'-Phosphate Decarboxylase

KW - Selection, Genetic

KW - Transformation, Genetic

U2 - 10.1007/BF00327197

DO - 10.1007/BF00327197

M3 - Article

C2 - 3481025

SN - 0026-8925

VL - 210

SP - 460

EP - 461

JO - Molecular and General Genetics

JF - Molecular and General Genetics

IS - 3

ER -

Transformation of Aspergillus oryzae using the A. niger pyrG gene

Abstract

Keywords

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