Transcriptional response to dietary restriction in Drosophila melanogaster

Feifei Ding, M. Pilar Gil, Michael Franklin, Jonathan Ferreira, Marc Tatar, Stephen L. Helfand, Nicola Neretti*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Dietary restriction (DR) extends lifespan in a wide variety of organisms. Although several genes and pathways associated with this longevity response have been identified, the specific mechanism through which DR extends lifespan is not fully understood. We have recently developed a novel methodology to screen for transcriptional changes in response to acutely imposed DR upon adult Drosophila melanogaster and identified groups of genes that switch their transcriptional patterns from a normal diet pattern to a restricted diet pattern, or 'switching genes'. In this current report we extend our transcriptional data analysis with gene set enrichment analysis to generate a pathway-centered perspective. The pattern of temporal behavior in response to the diet switch is strikingly similar within and across pathways associated with mRNA processing and protein translation. Furthermore, most genes within these pathways display an initial spike in activity within 6-8 h from the diet switch, followed by a coordinated, partial down-regulation after 24 h. We propose this represents a stereotypical response to DR, which ultimately leads to a mild but widespread inhibition of transcriptional and translational activity. Inhibition of the protein synthesis pathway has been observed in DR in other studies and has been shown to extend lifespan in several model organisms. (C) 2014 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalJournal of Insect Physiology
Volume69
DOIs
Publication statusPublished - Oct 2014

Keywords

  • Life span
  • Dietary restriction
  • Dietary switch
  • Gene expression
  • Pathway analysis
  • Drosophila melanogaster
  • LIFE-SPAN EXTENSION
  • CALORIC RESTRICTION
  • SIGNALING PATHWAY
  • RHESUS-MONKEYS
  • C-ELEGANS
  • GENES
  • TOR
  • PHOSPHORYLATION
  • MORTALITY
  • HEALTH

Fingerprint

Dive into the research topics of 'Transcriptional response to dietary restriction in Drosophila melanogaster'. Together they form a unique fingerprint.

Cite this