Transcription factor NFE2L1 decreases in glomerulonephropathies after podocyte damage

Mustafa Elshani; In Hwa Um; Steve Leung; Paul A. Reynolds; Alex Chapman; Mary Kudsy; David J. Harrison

doi:10.3390/cells12172165

Transcription factor NFE2L1 decreases in glomerulonephropathies after podocyte damage

Mustafa Elshani^*, In Hwa Um, Steve Leung, Paul A. Reynolds, Alex Chapman, Mary Kudsy, David J. Harrison

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Podocyte cellular injury and detachment from glomerular capillaries constitute a critical factor contributing to kidney disease. Notably, transcription factors are instrumental in maintaining podocyte differentiation and homeostasis. This study explores the hitherto uninvestigated expression of Nuclear Factor Erythroid 2-related Factor 1 (NFE2L1) in podocytes. We evaluated the podocyte expression of NFE2L1, Nuclear Factor Erythroid 2-related Factor 2 (NFE2L2), and NAD(P)H:quinone Oxidoreductase (NQO1) in 127 human glomerular disease biopsies using multiplexed immunofluorescence and image analysis. We found that both NFE2L1 and NQO1 expressions were significantly diminished across all observed renal diseases. Furthermore, we exposed human immortalized podocytes and ex vivo kidney slices to Puromycin Aminonucleoside (PAN) and characterized the NFE2L1 protein isoform expression. PAN treatment led to a reduction in the nuclear expression of NFE2L1 in ex vivo kidney slices and podocytes.

Original language	English
Article number	2165
Journal	Cells
Volume	12
Issue number	17
DOIs	https://doi.org/10.3390/cells12172165
Publication status	Published - 29 Aug 2023

Keywords

Podocytes
NFE2L1
NQO1
Glomerular disease
Transcription factor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/cells12172165Licence: CC BY

Elshani_2023_Cells_NFE2L1_CCBY
Copyright © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Final published version, 3.25 MBLicence: CC BY

https://www.mdpi.com/2073-4409/12/17/2165Licence: CC BY

KATY: KATY: Knowledge At the Tip of Your fingers: Clinical Knowledge for Humanity
Harrison, D. J. (PI)
European Commission
1/01/21 → 31/12/24
Project: Standard

Cite this

@article{deed01dd981e4adba6f5289264de76ad,

title = "Transcription factor NFE2L1 decreases in glomerulonephropathies after podocyte damage",

abstract = "Podocyte cellular injury and detachment from glomerular capillaries constitute a critical factor contributing to kidney disease. Notably, transcription factors are instrumental in maintaining podocyte differentiation and homeostasis. This study explores the hitherto uninvestigated expression of Nuclear Factor Erythroid 2-related Factor 1 (NFE2L1) in podocytes. We evaluated the podocyte expression of NFE2L1, Nuclear Factor Erythroid 2-related Factor 2 (NFE2L2), and NAD(P)H:quinone Oxidoreductase (NQO1) in 127 human glomerular disease biopsies using multiplexed immunofluorescence and image analysis. We found that both NFE2L1 and NQO1 expressions were significantly diminished across all observed renal diseases. Furthermore, we exposed human immortalized podocytes and ex vivo kidney slices to Puromycin Aminonucleoside (PAN) and characterized the NFE2L1 protein isoform expression. PAN treatment led to a reduction in the nuclear expression of NFE2L1 in ex vivo kidney slices and podocytes.",

keywords = "Podocytes, NFE2L1, NQO1, Glomerular disease, Transcription factor",

author = "Mustafa Elshani and Um, {In Hwa} and Steve Leung and Reynolds, {Paul A.} and Alex Chapman and Mary Kudsy and Harrison, {David J.}",

note = "Funding: This work was supported by NHS Lothian. This project received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation programme under Grant Agreement No. 101017453 as part of the KATY project, and from NuCana plc.",

year = "2023",

month = aug,

day = "29",

doi = "10.3390/cells12172165",

language = "English",

volume = "12",

journal = "Cells",

issn = "2073-4409",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "17",

}

TY - JOUR

T1 - Transcription factor NFE2L1 decreases in glomerulonephropathies after podocyte damage

AU - Elshani, Mustafa

AU - Um, In Hwa

AU - Leung, Steve

AU - Reynolds, Paul A.

AU - Chapman, Alex

AU - Kudsy, Mary

AU - Harrison, David J.

N1 - Funding: This work was supported by NHS Lothian. This project received funding from the European Union’s Horizon 2020 research and innovation programme under Grant Agreement No. 101017453 as part of the KATY project, and from NuCana plc.

PY - 2023/8/29

Y1 - 2023/8/29

N2 - Podocyte cellular injury and detachment from glomerular capillaries constitute a critical factor contributing to kidney disease. Notably, transcription factors are instrumental in maintaining podocyte differentiation and homeostasis. This study explores the hitherto uninvestigated expression of Nuclear Factor Erythroid 2-related Factor 1 (NFE2L1) in podocytes. We evaluated the podocyte expression of NFE2L1, Nuclear Factor Erythroid 2-related Factor 2 (NFE2L2), and NAD(P)H:quinone Oxidoreductase (NQO1) in 127 human glomerular disease biopsies using multiplexed immunofluorescence and image analysis. We found that both NFE2L1 and NQO1 expressions were significantly diminished across all observed renal diseases. Furthermore, we exposed human immortalized podocytes and ex vivo kidney slices to Puromycin Aminonucleoside (PAN) and characterized the NFE2L1 protein isoform expression. PAN treatment led to a reduction in the nuclear expression of NFE2L1 in ex vivo kidney slices and podocytes.

AB - Podocyte cellular injury and detachment from glomerular capillaries constitute a critical factor contributing to kidney disease. Notably, transcription factors are instrumental in maintaining podocyte differentiation and homeostasis. This study explores the hitherto uninvestigated expression of Nuclear Factor Erythroid 2-related Factor 1 (NFE2L1) in podocytes. We evaluated the podocyte expression of NFE2L1, Nuclear Factor Erythroid 2-related Factor 2 (NFE2L2), and NAD(P)H:quinone Oxidoreductase (NQO1) in 127 human glomerular disease biopsies using multiplexed immunofluorescence and image analysis. We found that both NFE2L1 and NQO1 expressions were significantly diminished across all observed renal diseases. Furthermore, we exposed human immortalized podocytes and ex vivo kidney slices to Puromycin Aminonucleoside (PAN) and characterized the NFE2L1 protein isoform expression. PAN treatment led to a reduction in the nuclear expression of NFE2L1 in ex vivo kidney slices and podocytes.

KW - Podocytes

KW - NFE2L1

KW - NQO1

KW - Glomerular disease

KW - Transcription factor

U2 - 10.3390/cells12172165

DO - 10.3390/cells12172165

M3 - Article

SN - 2073-4409

VL - 12

JO - Cells

JF - Cells

IS - 17

M1 - 2165

ER -

Transcription factor NFE2L1 decreases in glomerulonephropathies after podocyte damage

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Projects

KATY: KATY: Knowledge At the Tip of Your fingers: Clinical Knowledge for Humanity

Cite this