Transcription factor NFE2L1 decreases in glomerulonephropathies after podocyte damage

Mustafa Elshani*, In Hwa Um, Steve Leung, Paul A. Reynolds, Alex Chapman, Mary Kudsy, David J. Harrison

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Podocyte cellular injury and detachment from glomerular capillaries constitute a critical factor contributing to kidney disease. Notably, transcription factors are instrumental in maintaining podocyte differentiation and homeostasis. This study explores the hitherto uninvestigated expression of Nuclear Factor Erythroid 2-related Factor 1 (NFE2L1) in podocytes. We evaluated the podocyte expression of NFE2L1, Nuclear Factor Erythroid 2-related Factor 2 (NFE2L2), and NAD(P)H:quinone Oxidoreductase (NQO1) in 127 human glomerular disease biopsies using multiplexed immunofluorescence and image analysis. We found that both NFE2L1 and NQO1 expressions were significantly diminished across all observed renal diseases. Furthermore, we exposed human immortalized podocytes and ex vivo kidney slices to Puromycin Aminonucleoside (PAN) and characterized the NFE2L1 protein isoform expression. PAN treatment led to a reduction in the nuclear expression of NFE2L1 in ex vivo kidney slices and podocytes.
Original languageEnglish
Article number2165
JournalCells
Volume12
Issue number17
DOIs
Publication statusPublished - 29 Aug 2023

Keywords

  • Podocytes
  • NFE2L1
  • NQO1
  • Glomerular disease
  • Transcription factor

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