Trajectory-based co-localization measures for nanoparticle-cell interaction studies

Juan A Varela; Christoffer Åberg; Jeremy C Simpson; Kenneth A Dawson

doi:10.1002/smll.201401849

Trajectory-based co-localization measures for nanoparticle-cell interaction studies

Juan A Varela, Christoffer Åberg, Jeremy C Simpson, Kenneth A Dawson

School of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

High-resolution live cell microscopy will soon have a fundamental role in understanding bio-nano interactions, providing material that can be exploited using single particle tracking techniques. The present work uses 3D timelapse images obtained with confocal microscopy, to temporally resolve the co-localization between polystyrene nanoparticles and lysosomes in live cells through object-based measurements.

Original language	English
Pages (from-to)	2026-31
Number of pages	6
Journal	Small
Volume	11
Issue number	17
DOIs	https://doi.org/10.1002/smll.201401849
Publication status	Published - 6 May 2015

Keywords

Biological Transport
Cell Line, Tumor
Fluorescent Dyes/chemistry
Humans
Imaging, Three-Dimensional
Lung Neoplasms/metabolism
Lysosomes/chemistry
Microscopy, Confocal/instrumentation
Nanoparticles/chemistry
Polystyrenes/chemistry
Stochastic Processes
Time Factors

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10.1002/smll.201401849

Cite this

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title = "Trajectory-based co-localization measures for nanoparticle-cell interaction studies",

abstract = "High-resolution live cell microscopy will soon have a fundamental role in understanding bio-nano interactions, providing material that can be exploited using single particle tracking techniques. The present work uses 3D timelapse images obtained with confocal microscopy, to temporally resolve the co-localization between polystyrene nanoparticles and lysosomes in live cells through object-based measurements. ",

keywords = "Biological Transport, Cell Line, Tumor, Fluorescent Dyes/chemistry, Humans, Imaging, Three-Dimensional, Lung Neoplasms/metabolism, Lysosomes/chemistry, Microscopy, Confocal/instrumentation, Nanoparticles/chemistry, Polystyrenes/chemistry, Stochastic Processes, Time Factors",

author = "Varela, {Juan A} and Christoffer {\AA}berg and Simpson, {Jeremy C} and Dawson, {Kenneth A}",

note = "{\textcopyright} 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2015",

month = may,

day = "6",

doi = "10.1002/smll.201401849",

language = "English",

volume = "11",

pages = "2026--31",

journal = "Small",

issn = "1613-6810",

publisher = "Wiley",

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TY - JOUR

T1 - Trajectory-based co-localization measures for nanoparticle-cell interaction studies

AU - Varela, Juan A

AU - Åberg, Christoffer

AU - Simpson, Jeremy C

AU - Dawson, Kenneth A

PY - 2015/5/6

Y1 - 2015/5/6

N2 - High-resolution live cell microscopy will soon have a fundamental role in understanding bio-nano interactions, providing material that can be exploited using single particle tracking techniques. The present work uses 3D timelapse images obtained with confocal microscopy, to temporally resolve the co-localization between polystyrene nanoparticles and lysosomes in live cells through object-based measurements.

AB - High-resolution live cell microscopy will soon have a fundamental role in understanding bio-nano interactions, providing material that can be exploited using single particle tracking techniques. The present work uses 3D timelapse images obtained with confocal microscopy, to temporally resolve the co-localization between polystyrene nanoparticles and lysosomes in live cells through object-based measurements.

KW - Biological Transport

KW - Cell Line, Tumor

KW - Fluorescent Dyes/chemistry

KW - Humans

KW - Imaging, Three-Dimensional

KW - Lung Neoplasms/metabolism

KW - Lysosomes/chemistry

KW - Microscopy, Confocal/instrumentation

KW - Nanoparticles/chemistry

KW - Polystyrenes/chemistry

KW - Stochastic Processes

KW - Time Factors

U2 - 10.1002/smll.201401849

DO - 10.1002/smll.201401849

M3 - Article

C2 - 25504742

SN - 1613-6810

VL - 11

SP - 2026

EP - 2031

JO - Small

JF - Small

IS - 17

ER -

Trajectory-based co-localization measures for nanoparticle-cell interaction studies

Abstract

Keywords

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