Tracking the green invaders: advances in imaging virus infection in plants

Jens Tilsner, Karl J. Oparka

Research output: Contribution to journalReview articlepeer-review

19 Citations (Scopus)

Abstract

Bioimaging contributes significantly to our understanding of plant virus infections. In the present review, we describe technical advances that enable imaging of the infection process at previously unobtainable levels. We highlight how such new advances in subcellular imaging are contributing to a detailed dissection of all stages of the viral infection process. Specifically, we focus on: (i) the increasingly detailed localizations of viral proteins enabled by a diversifying palette of cellular markers; (ii) approaches using fluorescence microscopy for the functional analysis of proteins in vivo; (iii) the imaging of viral RNAs; (iv) methods that bridge the gap between optical and electron microscopy; and (v) methods that are blurring the distinction between imaging and structural biology. We describe the advantages and disadvantages of such techniques and place them in the broader perspective of their utility in analysing plant virus infection.

Original languageEnglish
Pages (from-to)21-37
Number of pages17
JournalBiochemical Journal
Volume430
DOIs
Publication statusPublished - 15 Aug 2010

Keywords

  • correlative microscopy
  • fluorescent protein
  • in vivo interaction
  • membrane topology
  • RNA imaging
  • super-resolution
  • BIMOLECULAR FLUORESCENCE COMPLEMENTATION
  • PROTEIN-PROTEIN INTERACTIONS
  • TRIPLE GENE BLOCK
  • RESONANCE ENERGY-TRANSFER
  • MESSENGER-RNA TRANSLATION
  • MAJOR NUCLEOLAR PROTEIN
  • GATEWAY BINARY VECTORS
  • TMV MOVEMENT PROTEIN
  • IN-VIVO FLUORESCENCE
  • STRIPE-MOSAIC-VIRUS

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