Abstract
Observing biological processes in real-time and in single live cells is a vital step
towards understanding cell behaviour and the way cells interact with the world
around them. However, this requires real time three dimensional (4D) tracking of
nanoparticles which is challenging and traditionally relies on sequential capture of
2D images to construct a 3D picture. We discuss a new approach to 4D
nanoparticle tracking that utilises a specially designed diffraction grating which
behaves as a lens with a different focal length in each diffraction order thereby
producing pseudo 3D imaging over the imaged field. The current experimental
system has the ability to track a single particle in a 50x50x6μm volume, with an
accuracy of better than 50 nm in each dimension.
towards understanding cell behaviour and the way cells interact with the world
around them. However, this requires real time three dimensional (4D) tracking of
nanoparticles which is challenging and traditionally relies on sequential capture of
2D images to construct a 3D picture. We discuss a new approach to 4D
nanoparticle tracking that utilises a specially designed diffraction grating which
behaves as a lens with a different focal length in each diffraction order thereby
producing pseudo 3D imaging over the imaged field. The current experimental
system has the ability to track a single particle in a 50x50x6μm volume, with an
accuracy of better than 50 nm in each dimension.
| Original language | English |
|---|---|
| Title of host publication | Adaptive Optics in Industry and Medicine Proceedings of the 6th international workshop |
| Editors | Christopher Dainty |
| Publisher | Imperial College Press |
| Pages | 394 |
| ISBN (Electronic) | 978-1-84816-111-5 |
| ISBN (Print) | 978-1-84816-110-8 |
| Publication status | Published - 2008 |