Tools for detection of Mycoplasma amphoriforme: a primary respiratory pathogen?

Clare L. Ling, Katarina Oravcova, Thomas Ferris Beattie, Dean D. Creer, Paul Dilworth, Naomi L. Fulton, Alison Hardie, Michelle Munro, Marcus Pond, Kate Templeton, David Webster, Sarita Workman, Timothy D. McHugh, Stephen Henry Gillespie

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Mycoplasma amphoriforme is a recently described organism isolated from the respiratory tracts of patients with immunodeficiency and evidence of chronic infection. Novel assays for the molecular detection of the organism by real-time quantitative PCRs (qPCRs) targeting the uracil DNA glycosylase gene (udg) or the 23S rRNA gene are described here. The analytical sensitivities are similar to the existing conventional M. amphoriforme 16S rRNA gene PCR, with the advantage of being species specific, rapid, and quantitative. By using these techniques, we demonstrate the presence of this organism in 17 (19.3%) primary antibody-deficient (PAD) patients, 4 (5%) adults with lower respiratory tract infection, 1 (2.6%) sputum sample from a patient attending a chest clinic, and 23 (0.21%) samples submitted for viral diagnosis of respiratory infection, but not in normal adult control subjects. These data show the presence of this microorganism in respiratory patients and suggest that M. amphoriforme may infect both immunocompetent and immunocompromised people. Further studies to characterize this organism are required, and this report provides the tools that may be used by other research groups to investigate its pathogenic potential.

Original languageEnglish
Pages (from-to)1177-1181
Number of pages5
JournalJournal of Clinical Microbiology
Volume52
Issue number4
Early online date1 Apr 2014
DOIs
Publication statusPublished - Apr 2014

Keywords

  • Mycoplasma
  • Pathogen discover
  • Lower respiratory tract infection

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