Tomato SlGSTU38 interacts with the PepMV coat protein and promotes viral infection

Eduardo Méndez-López; Livia Donaire; Blanca Gosálvez; Pedro Díaz-Vivancos; M. Amelia Sánchez-Pina; Jens Tilsner; Miguel A. Aranda

doi:10.1111/nph.18728

Tomato SlGSTU38 interacts with the PepMV coat protein and promotes viral infection

Eduardo Méndez-López^*, Livia Donaire, Blanca Gosálvez, Pedro Díaz-Vivancos, M. Amelia Sánchez-Pina, Jens Tilsner, Miguel A. Aranda

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Pepino mosaic virus (PepMV) is pandemic in tomato crops, causing important economic losses worldwide. No PepMV resistant varieties have been developed yet. Identification of host factors interacting with PepMV proteins is a promising source of genetic targets to develop PepMV resistant varieties.
The interaction between the PepMV coat protein (CP) and the tomato glutathione S-transferase SlGSTU38 was identified in a yeast-two hybrid (Y2H) screening and validated by directed Y2H and co-immunoprecipitation assays. SlGSTU38-knocked out Micro-Tom plants (gstu38) generated by the CRISPR/Cas9 technology together with live-cell imaging were used to understand the role of SlGSTU38 during infection. The transcriptomes of healthy and PepMV-infected wild type (WT) and gstu38 plants were profiled by RNA-seq analysis.
SlGSTU38 functions as a PepMV-specific susceptibility factor in a cell autonomous manner and re-localizes to the virus replication complexes during infection. Besides, knocking out SlGSTU38 triggers ROS accumulation in leaves and the deregulation of stress-responsive genes.
SlGSTU38 may play a dual role: on the one hand, SlGSTU38 may exert a proviral function depending on its specific interaction with the PepMV CP; and on the other hand, SlGSTU38 may delay sensing PepMV infection by participating in the redox intra-cellular homeostasis in a non-specific manner.

Original language	English
Pages (from-to)	332-348
Number of pages	17
Journal	New Phytologist
Volume	238
Issue number	1
Early online date	4 Feb 2023
DOIs	https://doi.org/10.1111/nph.18728
Publication status	Published - 1 Apr 2023

Keywords

PepMV
Potexvirus
Host factor
Redox homeostasis
Resistance
Stress response
Viral replication complex
VRC

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10.1111/nph.18728

Mendez-Lopez_2023_NP_Tomato-SIGSTU38_CC
Copyright © 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Final published version, 4.4 MBLicence: CC BY-NC-ND

Cite this

@article{cb98eb1224dd49949cb87911983d7426,

title = "Tomato SlGSTU38 interacts with the PepMV coat protein and promotes viral infection",

abstract = "Pepino mosaic virus (PepMV) is pandemic in tomato crops, causing important economic losses worldwide. No PepMV resistant varieties have been developed yet. Identification of host factors interacting with PepMV proteins is a promising source of genetic targets to develop PepMV resistant varieties. The interaction between the PepMV coat protein (CP) and the tomato glutathione S-transferase SlGSTU38 was identified in a yeast-two hybrid (Y2H) screening and validated by directed Y2H and co-immunoprecipitation assays. SlGSTU38-knocked out Micro-Tom plants (gstu38) generated by the CRISPR/Cas9 technology together with live-cell imaging were used to understand the role of SlGSTU38 during infection. The transcriptomes of healthy and PepMV-infected wild type (WT) and gstu38 plants were profiled by RNA-seq analysis. SlGSTU38 functions as a PepMV-specific susceptibility factor in a cell autonomous manner and re-localizes to the virus replication complexes during infection. Besides, knocking out SlGSTU38 triggers ROS accumulation in leaves and the deregulation of stress-responsive genes. SlGSTU38 may play a dual role: on the one hand, SlGSTU38 may exert a proviral function depending on its specific interaction with the PepMV CP; and on the other hand, SlGSTU38 may delay sensing PepMV infection by participating in the redox intra-cellular homeostasis in a non-specific manner.",

keywords = "PepMV, Potexvirus, Host factor, Redox homeostasis, Resistance, Stress response, Viral replication complex, VRC",

author = "Eduardo M{\'e}ndez-L{\'o}pez and Livia Donaire and Blanca Gos{\'a}lvez and Pedro D{\'i}az-Vivancos and S{\'a}nchez-Pina, {M. Amelia} and Jens Tilsner and Aranda, {Miguel A.}",

note = "Funding: This work was supported by grants RTI2018-097099-B-I00 and PID2021-125010OB-I00 from Ministerio de Ciencia e Innovaci{\'o}n (Spain). EM-L was supported by grant BES-2013-064540 (Ministerio de Ciencia e Innovaci{\'o}n; Spain). Work in JT laboratory was supported by the Scottish Government{\textquoteright}s Rural and Environment Science and Analytical Services Division (RESAS).",

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doi = "10.1111/nph.18728",

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volume = "238",

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journal = "New Phytologist",

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T1 - Tomato SlGSTU38 interacts with the PepMV coat protein and promotes viral infection

AU - Méndez-López, Eduardo

AU - Donaire, Livia

AU - Gosálvez, Blanca

AU - Díaz-Vivancos, Pedro

AU - Sánchez-Pina, M. Amelia

AU - Tilsner, Jens

AU - Aranda, Miguel A.

N1 - Funding: This work was supported by grants RTI2018-097099-B-I00 and PID2021-125010OB-I00 from Ministerio de Ciencia e Innovación (Spain). EM-L was supported by grant BES-2013-064540 (Ministerio de Ciencia e Innovación; Spain). Work in JT laboratory was supported by the Scottish Government’s Rural and Environment Science and Analytical Services Division (RESAS).

PY - 2023/4/1

Y1 - 2023/4/1

N2 - Pepino mosaic virus (PepMV) is pandemic in tomato crops, causing important economic losses worldwide. No PepMV resistant varieties have been developed yet. Identification of host factors interacting with PepMV proteins is a promising source of genetic targets to develop PepMV resistant varieties. The interaction between the PepMV coat protein (CP) and the tomato glutathione S-transferase SlGSTU38 was identified in a yeast-two hybrid (Y2H) screening and validated by directed Y2H and co-immunoprecipitation assays. SlGSTU38-knocked out Micro-Tom plants (gstu38) generated by the CRISPR/Cas9 technology together with live-cell imaging were used to understand the role of SlGSTU38 during infection. The transcriptomes of healthy and PepMV-infected wild type (WT) and gstu38 plants were profiled by RNA-seq analysis. SlGSTU38 functions as a PepMV-specific susceptibility factor in a cell autonomous manner and re-localizes to the virus replication complexes during infection. Besides, knocking out SlGSTU38 triggers ROS accumulation in leaves and the deregulation of stress-responsive genes. SlGSTU38 may play a dual role: on the one hand, SlGSTU38 may exert a proviral function depending on its specific interaction with the PepMV CP; and on the other hand, SlGSTU38 may delay sensing PepMV infection by participating in the redox intra-cellular homeostasis in a non-specific manner.

AB - Pepino mosaic virus (PepMV) is pandemic in tomato crops, causing important economic losses worldwide. No PepMV resistant varieties have been developed yet. Identification of host factors interacting with PepMV proteins is a promising source of genetic targets to develop PepMV resistant varieties. The interaction between the PepMV coat protein (CP) and the tomato glutathione S-transferase SlGSTU38 was identified in a yeast-two hybrid (Y2H) screening and validated by directed Y2H and co-immunoprecipitation assays. SlGSTU38-knocked out Micro-Tom plants (gstu38) generated by the CRISPR/Cas9 technology together with live-cell imaging were used to understand the role of SlGSTU38 during infection. The transcriptomes of healthy and PepMV-infected wild type (WT) and gstu38 plants were profiled by RNA-seq analysis. SlGSTU38 functions as a PepMV-specific susceptibility factor in a cell autonomous manner and re-localizes to the virus replication complexes during infection. Besides, knocking out SlGSTU38 triggers ROS accumulation in leaves and the deregulation of stress-responsive genes. SlGSTU38 may play a dual role: on the one hand, SlGSTU38 may exert a proviral function depending on its specific interaction with the PepMV CP; and on the other hand, SlGSTU38 may delay sensing PepMV infection by participating in the redox intra-cellular homeostasis in a non-specific manner.

KW - PepMV

KW - Potexvirus

KW - Host factor

KW - Redox homeostasis

KW - Resistance

KW - Stress response

KW - Viral replication complex

KW - VRC

U2 - 10.1111/nph.18728

DO - 10.1111/nph.18728

M3 - Article

SN - 0028-646X

VL - 238

SP - 332

EP - 348

JO - New Phytologist

JF - New Phytologist

IS - 1

ER -

Tomato SlGSTU38 interacts with the PepMV coat protein and promotes viral infection

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Keywords

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