Projects per year
Abstract
We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low photo-damage technique for fast volumetric imaging of biological samples. We extend this imaging modality to the three-photon regime enhancing its penetration depth. Our present study uses a standard conventional femtosecond pulsed laser at 1000 nm wavelength for the imaging of 450 μm diameter cellular spheroids. In addition, we show, experimentally and through numerical simulations, the potential advantages in three-photon light-sheet microscopy of using propagation-invariant Bessel beams in preference to Gaussian beams.
Original language | English |
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Pages (from-to) | 5484-5487 |
Number of pages | 4 |
Journal | Optics Letters |
Volume | 43 |
Issue number | 21 |
DOIs | |
Publication status | Published - 1 Nov 2018 |
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Dive into the research topics of 'Three-photon light-sheet fluorescence microscopy'. Together they form a unique fingerprint.Projects
- 3 Finished
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M Squared - USTAN Biophotonics Nexus: M Sqaured - St Andrews Biophotonics Nexus
Dholakia, K. (PI)
1/11/17 → 31/10/22
Project: Standard
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Resonant and shaped photonics for under: Resonant and shaped photonics for understanding the physical and biomedical world
Dholakia, K. (PI) & Gather, M. C. (CoI)
1/08/17 → 31/07/22
Project: Standard
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H2020 MSCA ITN - BE-OPTICAL: H2020 Marie Curie ITN 2015 BE-OPTICAL
Dholakia, K. (PI)
1/10/15 → 30/09/19
Project: Standard
Datasets
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Data underpinning: Three-photon light-sheet fluorescence microscopy
Nylk, J. (Creator), Gasparoli, F. M. (Creator), Escobet Montalban, A. (Creator), Yang, Z. (Creator), Dholakia, K. (Creator) & Liu, P. (Creator), University of St Andrews, 20 Jun 2023
DOI: 10.17630/ff413c1a-3670-427f-9f3f-670518b172b6
Dataset