Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards.

NG Crawford; J Zaldívar-Rae; C Hagen; A Schable; ER Rosenblum; Jefferson Alden Graves; TW Reeder; Michael Gordon Ritchie; TC Glenn

doi:10.1111/j.1471-8286.2007.01930.x

Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards.

NG Crawford, J Zaldívar-Rae, C Hagen, A Schable, ER Rosenblum, Jefferson Alden Graves, TW Reeder, Michael Gordon Ritchie, TC Glenn

Research output: Contribution to journal › Article › peer-review

Abstract

We describe polymerase chain reaction primers and amplification conditions for 13 microsatellite DNA loci isolated from two bisexual species of whiptail lizards Aspidoscelis costata huico and Aspidoscelis inornata. Primers were tested on either 16 or 48 individuals of A. c. huico and/or 26 individuals of A. inornata. Ten of the 13 primers were also tested against a panel of 31 additional whiptail taxa. We detected three to nine alleles per locus in A. c. huico and four to 19 alleles per locus in A. inornata, with observed heterozygosity ranging from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will be an important resource for surveys of genetic variation in these lizards.

Original language	English
Pages (from-to)	219-223
Number of pages	5
Journal	Molecular Ecology Resources
Volume	8
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-8286.2007.01930.x
Publication status	Published - Jan 2008

Keywords

Ameiva
Aspidoscelis
Cnemidophorus
lizard
microsatellite
PCR primers
SSR
STR
teiid
whiptail
TESSELATA
SELECTION
EVOLUTION
PRIMERS
MEXICO

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Crawford, NG., Zaldívar-Rae, J., Hagen, C., Schable, A., Rosenblum, ER., Graves, J. A., Reeder, TW., Ritchie, M. G., & Glenn, TC. (2008). Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards. Molecular Ecology Resources, 8(1), 219-223. https://doi.org/10.1111/j.1471-8286.2007.01930.x

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title = "Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards.",

abstract = "We describe polymerase chain reaction primers and amplification conditions for 13 microsatellite DNA loci isolated from two bisexual species of whiptail lizards Aspidoscelis costata huico and Aspidoscelis inornata. Primers were tested on either 16 or 48 individuals of A. c. huico and/or 26 individuals of A. inornata. Ten of the 13 primers were also tested against a panel of 31 additional whiptail taxa. We detected three to nine alleles per locus in A. c. huico and four to 19 alleles per locus in A. inornata, with observed heterozygosity ranging from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will be an important resource for surveys of genetic variation in these lizards.",

keywords = "Ameiva, Aspidoscelis, Cnemidophorus, lizard, microsatellite, PCR primers, SSR, STR, teiid, whiptail, TESSELATA, SELECTION, EVOLUTION, PRIMERS, MEXICO",

author = "NG Crawford and J Zald{\'i}var-Rae and C Hagen and A Schable and ER Rosenblum and Graves, {Jefferson Alden} and TW Reeder and Ritchie, {Michael Gordon} and TC Glenn",

year = "2008",

month = jan,

doi = "10.1111/j.1471-8286.2007.01930.x",

language = "English",

volume = "8",

pages = "219--223",

journal = "Molecular Ecology Resources",

issn = "1755-098X",

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Crawford, NG, Zaldívar-Rae, J, Hagen, C, Schable, A, Rosenblum, ER, Graves, JA, Reeder, TW, Ritchie, MG & Glenn, TC 2008, 'Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards.', Molecular Ecology Resources, vol. 8, no. 1, pp. 219-223. https://doi.org/10.1111/j.1471-8286.2007.01930.x

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AU - Crawford, NG

AU - Zaldívar-Rae, J

AU - Hagen, C

AU - Schable, A

AU - Rosenblum, ER

AU - Graves, Jefferson Alden

AU - Reeder, TW

AU - Ritchie, Michael Gordon

AU - Glenn, TC

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N2 - We describe polymerase chain reaction primers and amplification conditions for 13 microsatellite DNA loci isolated from two bisexual species of whiptail lizards Aspidoscelis costata huico and Aspidoscelis inornata. Primers were tested on either 16 or 48 individuals of A. c. huico and/or 26 individuals of A. inornata. Ten of the 13 primers were also tested against a panel of 31 additional whiptail taxa. We detected three to nine alleles per locus in A. c. huico and four to 19 alleles per locus in A. inornata, with observed heterozygosity ranging from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will be an important resource for surveys of genetic variation in these lizards.

AB - We describe polymerase chain reaction primers and amplification conditions for 13 microsatellite DNA loci isolated from two bisexual species of whiptail lizards Aspidoscelis costata huico and Aspidoscelis inornata. Primers were tested on either 16 or 48 individuals of A. c. huico and/or 26 individuals of A. inornata. Ten of the 13 primers were also tested against a panel of 31 additional whiptail taxa. We detected three to nine alleles per locus in A. c. huico and four to 19 alleles per locus in A. inornata, with observed heterozygosity ranging from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will be an important resource for surveys of genetic variation in these lizards.

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KW - lizard

KW - microsatellite

KW - PCR primers

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KW - STR

KW - teiid

KW - whiptail

KW - TESSELATA

KW - SELECTION

KW - EVOLUTION

KW - PRIMERS

KW - MEXICO

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DO - 10.1111/j.1471-8286.2007.01930.x

M3 - Article

SN - 1755-098X

VL - 8

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EP - 223

JO - Molecular Ecology Resources

JF - Molecular Ecology Resources

IS - 1

ER -

Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards.

Abstract

Keywords

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