Thirteen polymorphic microsatellite DNA loci from whiptails of the genus Aspidoscelis (Teiidae: Squamata) and related cnemidophorine lizards.

NG Crawford, J Zaldívar-Rae, C Hagen, A Schable, ER Rosenblum, Jefferson Alden Graves, TW Reeder, Michael Gordon Ritchie, TC Glenn

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We describe polymerase chain reaction primers and amplification conditions for 13 microsatellite DNA loci isolated from two bisexual species of whiptail lizards Aspidoscelis costata huico and Aspidoscelis inornata. Primers were tested on either 16 or 48 individuals of A. c. huico and/or 26 individuals of A. inornata. Ten of the 13 primers were also tested against a panel of 31 additional whiptail taxa. We detected three to nine alleles per locus in A. c. huico and four to 19 alleles per locus in A. inornata, with observed heterozygosity ranging from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will be an important resource for surveys of genetic variation in these lizards.

Original languageEnglish
Pages (from-to)219-223
Number of pages5
JournalMolecular Ecology Resources
Volume8
Issue number1
DOIs
Publication statusPublished - Jan 2008

Keywords

  • Ameiva
  • Aspidoscelis
  • Cnemidophorus
  • lizard
  • microsatellite
  • PCR primers
  • SSR
  • STR
  • teiid
  • whiptail
  • TESSELATA
  • SELECTION
  • EVOLUTION
  • PRIMERS
  • MEXICO

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