Third harmonic imaging contrast from tubular structures in the presence of index discontinuity

Joséphine Morizet; Nicolas Olivier; Pierre Mahou; Arthur Boutillon; Chiara Stringari; Emmanuel Beaurepaire

doi:10.1038/s41598-023-34528-7

Third harmonic imaging contrast from tubular structures in the presence of index discontinuity

Joséphine Morizet, Nicolas Olivier, Pierre Mahou, Arthur Boutillon, Chiara Stringari, Emmanuel Beaurepaire^*

^*Corresponding author for this work

School of Physics and Astronomy

Research output: Contribution to journal › Article › peer-review

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Abstract

Accurate interpretation of third harmonic generation (THG) microscopy images in terms of sample optical properties and microstructure is generally hampered by the presence of excitation field distortions resulting from sample heterogeneity. Numerical methods that account for these artifacts need to be established. In this work, we experimentally and numerically analyze the THG contrast obtained from stretched hollow glass pipettes embedded in different liquids. We also characterize the nonlinear optical properties of 2,2′-thiodiethanol (TDE), a water-soluble index-matching medium. We find that index discontinuity not only changes the level and modulation amplitude of polarization-resolved THG signals, but can even change the polarization direction producing maximum THG near interfaces. We then show that a finite-difference time-domain (FDTD) modeling strategy can accurately account for contrast observed in optically heterogeneous samples, whereas reference Fourier-based numerical approaches are accurate only in the absence of index mismatch. This work opens perspectives for interpreting THG microscopy images of tubular objects and other geometries.

Original language	English
Article number	7850
Number of pages	11
Journal	Scientific Reports
Volume	13
DOIs	https://doi.org/10.1038/s41598-023-34528-7
Publication status	Published - 15 May 2023

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Cite this

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title = "Third harmonic imaging contrast from tubular structures in the presence of index discontinuity",

abstract = "Accurate interpretation of third harmonic generation (THG) microscopy images in terms of sample optical properties and microstructure is generally hampered by the presence of excitation field distortions resulting from sample heterogeneity. Numerical methods that account for these artifacts need to be established. In this work, we experimentally and numerically analyze the THG contrast obtained from stretched hollow glass pipettes embedded in different liquids. We also characterize the nonlinear optical properties of 2,2′-thiodiethanol (TDE), a water-soluble index-matching medium. We find that index discontinuity not only changes the level and modulation amplitude of polarization-resolved THG signals, but can even change the polarization direction producing maximum THG near interfaces. We then show that a finite-difference time-domain (FDTD) modeling strategy can accurately account for contrast observed in optically heterogeneous samples, whereas reference Fourier-based numerical approaches are accurate only in the absence of index mismatch. This work opens perspectives for interpreting THG microscopy images of tubular objects and other geometries.",

author = "Jos{\'e}phine Morizet and Nicolas Olivier and Pierre Mahou and Arthur Boutillon and Chiara Stringari and Emmanuel Beaurepaire",

note = "Funding: Agence Nationale de la Recherche (ANR) (ANR-EQPX-0029, ANR-10-INBS-04, ANR-15-CE11-0012).",

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doi = "10.1038/s41598-023-34528-7",

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T1 - Third harmonic imaging contrast from tubular structures in the presence of index discontinuity

AU - Morizet, Joséphine

AU - Olivier, Nicolas

AU - Mahou, Pierre

AU - Boutillon, Arthur

AU - Stringari, Chiara

AU - Beaurepaire, Emmanuel

N1 - Funding: Agence Nationale de la Recherche (ANR) (ANR-EQPX-0029, ANR-10-INBS-04, ANR-15-CE11-0012).

PY - 2023/5/15

Y1 - 2023/5/15

N2 - Accurate interpretation of third harmonic generation (THG) microscopy images in terms of sample optical properties and microstructure is generally hampered by the presence of excitation field distortions resulting from sample heterogeneity. Numerical methods that account for these artifacts need to be established. In this work, we experimentally and numerically analyze the THG contrast obtained from stretched hollow glass pipettes embedded in different liquids. We also characterize the nonlinear optical properties of 2,2′-thiodiethanol (TDE), a water-soluble index-matching medium. We find that index discontinuity not only changes the level and modulation amplitude of polarization-resolved THG signals, but can even change the polarization direction producing maximum THG near interfaces. We then show that a finite-difference time-domain (FDTD) modeling strategy can accurately account for contrast observed in optically heterogeneous samples, whereas reference Fourier-based numerical approaches are accurate only in the absence of index mismatch. This work opens perspectives for interpreting THG microscopy images of tubular objects and other geometries.

AB - Accurate interpretation of third harmonic generation (THG) microscopy images in terms of sample optical properties and microstructure is generally hampered by the presence of excitation field distortions resulting from sample heterogeneity. Numerical methods that account for these artifacts need to be established. In this work, we experimentally and numerically analyze the THG contrast obtained from stretched hollow glass pipettes embedded in different liquids. We also characterize the nonlinear optical properties of 2,2′-thiodiethanol (TDE), a water-soluble index-matching medium. We find that index discontinuity not only changes the level and modulation amplitude of polarization-resolved THG signals, but can even change the polarization direction producing maximum THG near interfaces. We then show that a finite-difference time-domain (FDTD) modeling strategy can accurately account for contrast observed in optically heterogeneous samples, whereas reference Fourier-based numerical approaches are accurate only in the absence of index mismatch. This work opens perspectives for interpreting THG microscopy images of tubular objects and other geometries.

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VL - 13

JO - Scientific Reports

JF - Scientific Reports

M1 - 7850

ER -

Third harmonic imaging contrast from tubular structures in the presence of index discontinuity

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