Abstract
Reduction of HAuCl4 by NaSCN or KSCN produces colloidal gold particles of 2.6 nm in diameter and homogeneous in size (coefficient of variation approximately 15%). The AuSCN sol forms protein-gold complexes. The amount of protein required to form an AuSCN-protein complex is best determined in the electron microscope, where serial dilutions of protein with gold sol are inspected for the presence of aggregates. By immuno-electron microscopy SCN-gold complexed to protein A is active and visible as is shown by revealing alpha-amylase in rat pancreatic acinar cells.
Original language | English |
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Pages (from-to) | 409-411 |
Number of pages | 3 |
Journal | Histochemistry |
Volume | 83 |
Issue number | 5 |
DOIs | |
Publication status | Published - 1985 |
Keywords
- Animals
- Colloids
- Gold
- Histocytochemistry
- Male
- Microscopy, Electron
- Pancreas
- Rats
- Rats, Inbred Strains
- Staphylococcal Protein A
- Thiocyanates
- alpha-Amylases