"Thiocyanate gold": small (2-3 nm) colloidal gold for affinity cytochemical labeling in electron microscopy

W Baschong, J M Lucocq, J Roth

Research output: Contribution to journalArticlepeer-review

106 Citations (Scopus)

Abstract

Reduction of HAuCl4 by NaSCN or KSCN produces colloidal gold particles of 2.6 nm in diameter and homogeneous in size (coefficient of variation approximately 15%). The AuSCN sol forms protein-gold complexes. The amount of protein required to form an AuSCN-protein complex is best determined in the electron microscope, where serial dilutions of protein with gold sol are inspected for the presence of aggregates. By immuno-electron microscopy SCN-gold complexed to protein A is active and visible as is shown by revealing alpha-amylase in rat pancreatic acinar cells.
Original languageEnglish
Pages (from-to)409-411
Number of pages3
JournalHistochemistry
Volume83
Issue number5
DOIs
Publication statusPublished - 1985

Keywords

  • Animals
  • Colloids
  • Gold
  • Histocytochemistry
  • Male
  • Microscopy, Electron
  • Pancreas
  • Rats
  • Rats, Inbred Strains
  • Staphylococcal Protein A
  • Thiocyanates
  • alpha-Amylases

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