The V proteins of simian virus 5 and other paramyxoviruses inhibit induction of interferon-beta

E Poole, B He, RA Lamb, Richard Edward Randall, S Goodbourn

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170 Citations (Scopus)

Abstract

In this article we show that the paramyxovirus SV5 is a poor inducer of interferon-beta (IFN-beta). This inefficient induction is a consequence of the expression of an intact viral V protein. In the absence of the viral V protein cysteine-rich C-terminal domain, IFN-beta mRNA is strongly induced and the transcription factors NF-kappaB and IRF-3 are activated significantly The V protein can work in isolation from SV5 to block intracellular dsRNA signaling. The mechanism of block to dsRNA signaling is distinct from that previously observed for blocking IFN signaling in that proteolysis of candidate factors cannot be detected, and furthermore, the respective blocks require distinct protein domains. Blocking of the induction of IFN-beta by dsRNA requires the C-terminal cysteine-rich domain, a feature that is highly conserved among paramyxoviruses. We demonstrate that the V proteins from other paramyxoviruses have equivalent functions and speculate that limiting the yield of IFN-beta during infection may be a general property of paramyxoviruses. (C) 2002 Elsevier Science (USA).

Original languageEnglish
Pages (from-to)33-46
Number of pages14
JournalVirology
Volume303
Issue number1
DOIs
Publication statusPublished - 10 Nov 2002

Keywords

  • DOUBLE-STRANDED-RNA
  • NF-KAPPA-B
  • RECOMBINANT MEASLES VIRUSES
  • EMBRYONAL CARCINOMA-CELLS
  • SENDAI-VIRUS
  • REGULATORY FACTOR-3
  • IN-VIVO
  • TRANSCRIPTION FACTORS
  • VIRAL PATHOGENESIS
  • GENE-EXPRESSION

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