The V protein of Simian Virus 5 inhibits interferon signalling by targeting STAT1 for proteasome-mediated degradation

L Didcock, DF Young, S Goodbourn, Richard Edward Randall

Research output: Contribution to journalArticlepeer-review

378 Citations (Scopus)

Abstract

To replicate in vivo, viruses must circumvent cellular antiviral defense mechanisms, including those induced by the interferons (IFNs). Here we demonstrate that simian virus 5 (SV5) blocks IFN signalling in human tells by inhibiting the formation of the IFN-stimulated gene factor 3 and gamma-activated factor transcription complexes that are involved in activating IFN-alpha/beta- and IFN-gamma-responsive genes, respectively. SV5 inhibits the formation of these complexes by specifically targeting SV5, a component common to both transcription complexes, for proteasome-mediated degradation. Expression of the SV5 structural protein V, in the absence of other virus proteins, also inhibited IFN signalling and induced the degradation of STAT1. Following infection with SV5, STAT1 was degraded in the absence of virus protein synthesis and remained undetectable for up to 4 days postinfection, Furthermore, STAT1 was also degraded in IFN-pretreated cells, even though the cells were in an antiviral state. Since pretreatment of cells with HIV delayed but did not prevent virus replication and protein synthesis, these observations suggest that following infection of IFN-pretreated cells, SV5 remains viable within the cells until they eventually go out of the antiviral state.

Original languageEnglish
Pages (from-to)9928-9933
Number of pages6
JournalJournal of Virology
Volume73
Publication statusPublished - Dec 1999

Keywords

  • NF-KAPPA-B
  • HUMAN PAPILLOMAVIRUS TYPE-16
  • MHC CLASS-I
  • SIGNALING PATHWAY
  • ALPHA-INTERFERON
  • E6 ONCOPROTEIN
  • SENDAI-VIRUS
  • NP-P
  • PARAMYXOVIRUS
  • ACTIVATION

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