The use of composite pulses for improving DEER signal at 94 GHz

Claire L. Motion; Scott L. Cassidy; Paul A. S. Cruickshank; Robert I. Hunter; David R. Bolton; Hassane El Mkami; Sabine Van Doorslaer; Janet E. Lovett; Graham M. Smith

doi:10.1016/j.jmr.2017.03.018

The use of composite pulses for improving DEER signal at 94 GHz

Claire L. Motion, Scott L. Cassidy, Paul A. S. Cruickshank, Robert I. Hunter, David R. Bolton, Hassane El Mkami, Sabine Van Doorslaer, Janet E. Lovett, Graham M. Smith

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

10 Downloads (Pure)

Abstract

The sensitivity of pulsed electron paramagnetic resonance (EPR) measurements on broad-line paramagnetic centers is often limited by the available excitation bandwidth. One way to increase excitation bandwidth is through the use of chirp or composite pulses. However, performance can be limited by cavity or detection bandwidth, which in commercial systems is typically 100-200 MHz. Here we demonstrate in a 94 GHz spectrometer, with > 800 MHz system bandwidth, an increase in signal and modulation depth in a 4-pulse DEER experiment through use of composite rather than rectangular π pulses. We show that this leads to an increase in sensitivity by a factor of 3, in line with theoretical predictions, although gains are more limited in nitroxide-nitroxide DEER measurements.

Original language	English
Pages (from-to)	122-133
Number of pages	12
Journal	Journal of Magnetic Resonance
Volume	278
Early online date	2 Apr 2017
DOIs	https://doi.org/10.1016/j.jmr.2017.03.018
Publication status	Published - May 2017

Keywords

Composite pulses
Sensitivity enhancement
Echo simulation
DEER
PELDOR
Broadband non-resonant
W-band
Instrumentation

Access to Document

10.1016/j.jmr.2017.03.018

Smith_2017_JMR_CompositePulses_Updated_AAM
© 2017 Elsevier Ltd. All rights reserved. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.jmr.2017.03.018
Accepted author manuscript, 1.88 MBLicence: CC BY-NC-ND

Cite this

@article{d21a9c0277ef4b2d94df223b1bf282eb,

title = "The use of composite pulses for improving DEER signal at 94 GHz",

abstract = "The sensitivity of pulsed electron paramagnetic resonance (EPR) measurements on broad-line paramagnetic centers is often limited by the available excitation bandwidth. One way to increase excitation bandwidth is through the use of chirp or composite pulses. However, performance can be limited by cavity or detection bandwidth, which in commercial systems is typically 100-200 MHz. Here we demonstrate in a 94 GHz spectrometer, with > 800 MHz system bandwidth, an increase in signal and modulation depth in a 4-pulse DEER experiment through use of composite rather than rectangular π pulses. We show that this leads to an increase in sensitivity by a factor of 3, in line with theoretical predictions, although gains are more limited in nitroxide-nitroxide DEER measurements.",

keywords = "Composite pulses, Sensitivity enhancement, Echo simulation, DEER, PELDOR, Broadband non-resonant, W-band, Instrumentation",

author = "Motion, {Claire L.} and Cassidy, {Scott L.} and Cruickshank, {Paul A. S.} and Hunter, {Robert I.} and Bolton, {David R.} and {El Mkami}, Hassane and {Van Doorslaer}, Sabine and Lovett, {Janet E.} and Smith, {Graham M.}",

note = "C.L.M. acknowledges funding from EPSRC as part of the iMRCDT. The W-band instrument was developed under the U.K. Research Councils Basic Technology Program (grant EP/F039034/1). S.V.D. acknowledges the Research Foundation Flanders (FWO) for financial support (grant G.0687.13). J.E.L. thanks the Royal Society for a University Research Fellowship. Sylvia Dewilde (Biomedical Sciences, University of Antwerp) is thanked for the purification of the Cys46Ser/ Cys55Ser mutant of human neuroglobin (NGB) used in this work. Adelheid Godt{\textquoteright}s group is thanked for the synthesis of MSA236. We also thank the Wellcome Trust (grant 099149/Z/12/Z). The research data (and/or materials) supporting this publication can be accessed at http://dx.doi.org/10.17630/b65d05e6-6efa-48b9-a741-5a6322159a4a.",

year = "2017",

month = may,

doi = "10.1016/j.jmr.2017.03.018",

language = "English",

volume = "278",

pages = "122--133",

journal = "Journal of Magnetic Resonance",

issn = "1090-7807",

publisher = "Academic Press/Elsevier ",

}

TY - JOUR

T1 - The use of composite pulses for improving DEER signal at 94 GHz

AU - Motion, Claire L.

AU - Cassidy, Scott L.

AU - Cruickshank, Paul A. S.

AU - Hunter, Robert I.

AU - Bolton, David R.

AU - El Mkami, Hassane

AU - Van Doorslaer, Sabine

AU - Lovett, Janet E.

AU - Smith, Graham M.

N1 - C.L.M. acknowledges funding from EPSRC as part of the iMRCDT. The W-band instrument was developed under the U.K. Research Councils Basic Technology Program (grant EP/F039034/1). S.V.D. acknowledges the Research Foundation Flanders (FWO) for financial support (grant G.0687.13). J.E.L. thanks the Royal Society for a University Research Fellowship. Sylvia Dewilde (Biomedical Sciences, University of Antwerp) is thanked for the purification of the Cys46Ser/ Cys55Ser mutant of human neuroglobin (NGB) used in this work. Adelheid Godt’s group is thanked for the synthesis of MSA236. We also thank the Wellcome Trust (grant 099149/Z/12/Z). The research data (and/or materials) supporting this publication can be accessed at http://dx.doi.org/10.17630/b65d05e6-6efa-48b9-a741-5a6322159a4a.

PY - 2017/5

Y1 - 2017/5

N2 - The sensitivity of pulsed electron paramagnetic resonance (EPR) measurements on broad-line paramagnetic centers is often limited by the available excitation bandwidth. One way to increase excitation bandwidth is through the use of chirp or composite pulses. However, performance can be limited by cavity or detection bandwidth, which in commercial systems is typically 100-200 MHz. Here we demonstrate in a 94 GHz spectrometer, with > 800 MHz system bandwidth, an increase in signal and modulation depth in a 4-pulse DEER experiment through use of composite rather than rectangular π pulses. We show that this leads to an increase in sensitivity by a factor of 3, in line with theoretical predictions, although gains are more limited in nitroxide-nitroxide DEER measurements.

AB - The sensitivity of pulsed electron paramagnetic resonance (EPR) measurements on broad-line paramagnetic centers is often limited by the available excitation bandwidth. One way to increase excitation bandwidth is through the use of chirp or composite pulses. However, performance can be limited by cavity or detection bandwidth, which in commercial systems is typically 100-200 MHz. Here we demonstrate in a 94 GHz spectrometer, with > 800 MHz system bandwidth, an increase in signal and modulation depth in a 4-pulse DEER experiment through use of composite rather than rectangular π pulses. We show that this leads to an increase in sensitivity by a factor of 3, in line with theoretical predictions, although gains are more limited in nitroxide-nitroxide DEER measurements.

KW - Composite pulses

KW - Sensitivity enhancement

KW - Echo simulation

KW - DEER

KW - PELDOR

KW - Broadband non-resonant

KW - W-band

KW - Instrumentation

UR - http://www.sciencedirect.com/science/article/pii/S1090780717300903#appd003

U2 - 10.1016/j.jmr.2017.03.018

DO - 10.1016/j.jmr.2017.03.018

M3 - Article

SN - 1090-7807

VL - 278

SP - 122

EP - 133

JO - Journal of Magnetic Resonance

JF - Journal of Magnetic Resonance

ER -

The use of composite pulses for improving DEER signal at 94 GHz

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Royal Society Research Fellowship: Royal Society Research Fellowship

State of the art pulse EPR instrumentati: State of the art pulse EPR instrumentation for long range distance measurements in biomacromolecules

EP/F0390034/1 The HIPER Project: Bringing the NMR Paradigm to ESR - The HIPER Project

Data underpinning - The use of Composite Pulses for improving DEER signal at 94 GHz

Cite this

The use of composite pulses for improving DEER signal at 94 GHz

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

Royal Society Research Fellowship: Royal Society Research Fellowship

State of the art pulse EPR instrumentati: State of the art pulse EPR instrumentation for long range distance measurements in biomacromolecules

EP/F0390034/1 The HIPER Project: Bringing the NMR Paradigm to ESR - The HIPER Project

Datasets

Data underpinning - The use of Composite Pulses for improving DEER signal at 94 GHz

Cite this