The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services

V Vincent, L Rigouts, E Nduwamahoro, B Holmes, J Cunningham, M Guillerm, C-M Nathanson, F Moussy, B De Jong, F Portaels, A Ramsay

Research output: Contribution to journalArticlepeer-review


BACKGROUND: The Special Programme for Research and Training in Tropical Diseases recently launched a Mycobacterium tuberculosis strain bank (TDR-TB Strain Bank).

OBJECTIVE: To describe the TDR-TB Strain Bank, the characterisation of strains, bank management and the procedure for releasing materials.

RESULTS: The TDR-TB Strain Bank consists of 229 clinical M. tuberculosis isolates (single-colony derived cultures) plus five mycobacterial reference strains for purposes of identification. These are available as freeze-dried, viable strains or as heat-inactivated bacterial suspensions, quality controlled for purity, viability and authenticity. Isolates originated from diverse geographical settings and were selected for their resistance profiles against first- and second-line drugs. Low and high levels of resistance were determined by the minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol, streptomycin, ofloxacin, kanamycin, capreomycin, ethionamide and para-aminosalicylic acid. Sequencing for drug resistance mutations was performed on the relevant sections of the rpoB, katG, inhA, embB, rpsL, rrs, gyrA and gyrB genes. Typing using lineage-defining loci of mycobacterial interspersed repetitive unit-variable number tandem repeats indicated that the most important genetic lineages were represented.

CONCLUSIONS: The TDR-TB Strain Bank is a high quality bioresource for basic science, supporting the development of new diagnostics and drug-resistant detection tools and providing reference materials for laboratory quality management programmes.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalInternational Journal of Tuberculosis and Lung Disease
Issue number1
Publication statusPublished - Jan 2012


  • Antitubercular Agents/therapeutic use
  • Biological Specimen Banks/organization & administration
  • DNA Mutational Analysis
  • Drug Resistance, Multiple, Bacterial/genetics
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Diagnostic Techniques
  • Mutation
  • Mycobacterium tuberculosis/classification
  • Predictive Value of Tests
  • Program Development
  • Tuberculosis/diagnosis


Dive into the research topics of 'The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services'. Together they form a unique fingerprint.

Cite this