The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services

V Vincent; L Rigouts; E Nduwamahoro; B Holmes; J Cunningham; M Guillerm; C-M Nathanson; F Moussy; B De Jong; F Portaels; A Ramsay

doi:10.5588/ijtld.11.0223

The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services

V Vincent, L Rigouts, E Nduwamahoro, B Holmes, J Cunningham, M Guillerm, C-M Nathanson, F Moussy, B De Jong, F Portaels, A Ramsay

School of Medicine

Research output: Contribution to journal › Article › peer-review

Abstract

BACKGROUND: The Special Programme for Research and Training in Tropical Diseases recently launched a Mycobacterium tuberculosis strain bank (TDR-TB Strain Bank).

OBJECTIVE: To describe the TDR-TB Strain Bank, the characterisation of strains, bank management and the procedure for releasing materials.

RESULTS: The TDR-TB Strain Bank consists of 229 clinical M. tuberculosis isolates (single-colony derived cultures) plus five mycobacterial reference strains for purposes of identification. These are available as freeze-dried, viable strains or as heat-inactivated bacterial suspensions, quality controlled for purity, viability and authenticity. Isolates originated from diverse geographical settings and were selected for their resistance profiles against first- and second-line drugs. Low and high levels of resistance were determined by the minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol, streptomycin, ofloxacin, kanamycin, capreomycin, ethionamide and para-aminosalicylic acid. Sequencing for drug resistance mutations was performed on the relevant sections of the rpoB, katG, inhA, embB, rpsL, rrs, gyrA and gyrB genes. Typing using lineage-defining loci of mycobacterial interspersed repetitive unit-variable number tandem repeats indicated that the most important genetic lineages were represented.

CONCLUSIONS: The TDR-TB Strain Bank is a high quality bioresource for basic science, supporting the development of new diagnostics and drug-resistant detection tools and providing reference materials for laboratory quality management programmes.

Original language	English
Pages (from-to)	24-31
Number of pages	8
Journal	International Journal of Tuberculosis and Lung Disease
Volume	16
Issue number	1
DOIs	https://doi.org/10.5588/ijtld.11.0223
Publication status	Published - Jan 2012

Keywords

Antitubercular Agents/therapeutic use
Biological Specimen Banks/organization & administration
DNA Mutational Analysis
Drug Resistance, Multiple, Bacterial/genetics
Humans
Microbial Sensitivity Tests
Molecular Diagnostic Techniques
Mutation
Mycobacterium tuberculosis/classification
Predictive Value of Tests
Program Development
Tuberculosis/diagnosis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5588/ijtld.11.0223

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Vincent, V., Rigouts, L., Nduwamahoro, E., Holmes, B., Cunningham, J., Guillerm, M., Nathanson, C.-M., Moussy, F., De Jong, B., Portaels, F., & Ramsay, A. (2012). The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services. International Journal of Tuberculosis and Lung Disease, 16(1), 24-31. https://doi.org/10.5588/ijtld.11.0223

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title = "The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services",

abstract = "BACKGROUND: The Special Programme for Research and Training in Tropical Diseases recently launched a Mycobacterium tuberculosis strain bank (TDR-TB Strain Bank).OBJECTIVE: To describe the TDR-TB Strain Bank, the characterisation of strains, bank management and the procedure for releasing materials.RESULTS: The TDR-TB Strain Bank consists of 229 clinical M. tuberculosis isolates (single-colony derived cultures) plus five mycobacterial reference strains for purposes of identification. These are available as freeze-dried, viable strains or as heat-inactivated bacterial suspensions, quality controlled for purity, viability and authenticity. Isolates originated from diverse geographical settings and were selected for their resistance profiles against first- and second-line drugs. Low and high levels of resistance were determined by the minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol, streptomycin, ofloxacin, kanamycin, capreomycin, ethionamide and para-aminosalicylic acid. Sequencing for drug resistance mutations was performed on the relevant sections of the rpoB, katG, inhA, embB, rpsL, rrs, gyrA and gyrB genes. Typing using lineage-defining loci of mycobacterial interspersed repetitive unit-variable number tandem repeats indicated that the most important genetic lineages were represented.CONCLUSIONS: The TDR-TB Strain Bank is a high quality bioresource for basic science, supporting the development of new diagnostics and drug-resistant detection tools and providing reference materials for laboratory quality management programmes.",

keywords = "Antitubercular Agents/therapeutic use, Biological Specimen Banks/organization & administration, DNA Mutational Analysis, Drug Resistance, Multiple, Bacterial/genetics, Humans, Microbial Sensitivity Tests, Molecular Diagnostic Techniques, Mutation, Mycobacterium tuberculosis/classification, Predictive Value of Tests, Program Development, Tuberculosis/diagnosis",

author = "V Vincent and L Rigouts and E Nduwamahoro and B Holmes and J Cunningham and M Guillerm and C-M Nathanson and F Moussy and {De Jong}, B and F Portaels and A Ramsay",

year = "2012",

month = jan,

doi = "10.5588/ijtld.11.0223",

language = "English",

volume = "16",

pages = "24--31",

journal = "International Journal of Tuberculosis and Lung Disease",

issn = "1027-3719",

publisher = "International Union against Tubercul. and Lung Dis.",

number = "1",

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Vincent, V, Rigouts, L, Nduwamahoro, E, Holmes, B, Cunningham, J, Guillerm, M, Nathanson, C-M, Moussy, F, De Jong, B, Portaels, F & Ramsay, A 2012, 'The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services', International Journal of Tuberculosis and Lung Disease, vol. 16, no. 1, pp. 24-31. https://doi.org/10.5588/ijtld.11.0223

TY - JOUR

T1 - The TDR Tuberculosis Strain Bank

T2 - a resource for basic science, tool development and diagnostic services

AU - Vincent, V

AU - Rigouts, L

AU - Nduwamahoro, E

AU - Holmes, B

AU - Cunningham, J

AU - Guillerm, M

AU - Nathanson, C-M

AU - Moussy, F

AU - De Jong, B

AU - Portaels, F

AU - Ramsay, A

PY - 2012/1

Y1 - 2012/1

N2 - BACKGROUND: The Special Programme for Research and Training in Tropical Diseases recently launched a Mycobacterium tuberculosis strain bank (TDR-TB Strain Bank).OBJECTIVE: To describe the TDR-TB Strain Bank, the characterisation of strains, bank management and the procedure for releasing materials.RESULTS: The TDR-TB Strain Bank consists of 229 clinical M. tuberculosis isolates (single-colony derived cultures) plus five mycobacterial reference strains for purposes of identification. These are available as freeze-dried, viable strains or as heat-inactivated bacterial suspensions, quality controlled for purity, viability and authenticity. Isolates originated from diverse geographical settings and were selected for their resistance profiles against first- and second-line drugs. Low and high levels of resistance were determined by the minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol, streptomycin, ofloxacin, kanamycin, capreomycin, ethionamide and para-aminosalicylic acid. Sequencing for drug resistance mutations was performed on the relevant sections of the rpoB, katG, inhA, embB, rpsL, rrs, gyrA and gyrB genes. Typing using lineage-defining loci of mycobacterial interspersed repetitive unit-variable number tandem repeats indicated that the most important genetic lineages were represented.CONCLUSIONS: The TDR-TB Strain Bank is a high quality bioresource for basic science, supporting the development of new diagnostics and drug-resistant detection tools and providing reference materials for laboratory quality management programmes.

AB - BACKGROUND: The Special Programme for Research and Training in Tropical Diseases recently launched a Mycobacterium tuberculosis strain bank (TDR-TB Strain Bank).OBJECTIVE: To describe the TDR-TB Strain Bank, the characterisation of strains, bank management and the procedure for releasing materials.RESULTS: The TDR-TB Strain Bank consists of 229 clinical M. tuberculosis isolates (single-colony derived cultures) plus five mycobacterial reference strains for purposes of identification. These are available as freeze-dried, viable strains or as heat-inactivated bacterial suspensions, quality controlled for purity, viability and authenticity. Isolates originated from diverse geographical settings and were selected for their resistance profiles against first- and second-line drugs. Low and high levels of resistance were determined by the minimum inhibitory concentrations of isoniazid, rifampicin, ethambutol, streptomycin, ofloxacin, kanamycin, capreomycin, ethionamide and para-aminosalicylic acid. Sequencing for drug resistance mutations was performed on the relevant sections of the rpoB, katG, inhA, embB, rpsL, rrs, gyrA and gyrB genes. Typing using lineage-defining loci of mycobacterial interspersed repetitive unit-variable number tandem repeats indicated that the most important genetic lineages were represented.CONCLUSIONS: The TDR-TB Strain Bank is a high quality bioresource for basic science, supporting the development of new diagnostics and drug-resistant detection tools and providing reference materials for laboratory quality management programmes.

KW - Antitubercular Agents/therapeutic use

KW - Biological Specimen Banks/organization & administration

KW - DNA Mutational Analysis

KW - Drug Resistance, Multiple, Bacterial/genetics

KW - Humans

KW - Microbial Sensitivity Tests

KW - Molecular Diagnostic Techniques

KW - Mutation

KW - Mycobacterium tuberculosis/classification

KW - Predictive Value of Tests

KW - Program Development

KW - Tuberculosis/diagnosis

U2 - 10.5588/ijtld.11.0223

DO - 10.5588/ijtld.11.0223

M3 - Article

C2 - 22236841

SN - 1027-3719

VL - 16

SP - 24

EP - 31

JO - International Journal of Tuberculosis and Lung Disease

JF - International Journal of Tuberculosis and Lung Disease

IS - 1

ER -

The TDR Tuberculosis Strain Bank: a resource for basic science, tool development and diagnostic services

Abstract

Keywords

UN SDGs

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