The synthesis of EPR differentiable spinlabels and their coupling to uridine

Olga Frolow, Bela E. Bode, Joachim W. Angels

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

For EPR measurements of RNA, DNA, or proteins, the occurrence of the paramagnetic species is necessary. The aim of this work is to improve the synthesis of two different EPR spinlabels 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-N-oxyl-4-acetylene (TEMPA) 6 and (15) N-labeled TEMPA 6* and their coupling to uridine. The yield of the synthesis of TEMPA could be increased to 40 % and the second nitroxide 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-(15) N-oxyl-4-acetylene 6* could be synthesized with a yield of 11 %.

Original languageEnglish
Pages (from-to)655-659
Number of pages5
JournalNucleosides, Nucleotides and Nucleic Acids
Volume26
Issue number6-7
DOIs
Publication statusPublished - 2007

Keywords

  • RNA structure
  • sonogashira cross-coupling
  • side-specific label
  • nitroxide
  • EPR
  • NITROXIDE
  • PELDOR

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