The synthesis of EPR differentiable spinlabels and their coupling to uridine

Olga Frolow; Bela E. Bode; Joachim W. Angels

doi:10.1080/15257770701490522

The synthesis of EPR differentiable spinlabels and their coupling to uridine

Olga Frolow, Bela E. Bode, Joachim W. Angels

Research output: Contribution to journal › Article › peer-review

Abstract

For EPR measurements of RNA, DNA, or proteins, the occurrence of the paramagnetic species is necessary. The aim of this work is to improve the synthesis of two different EPR spinlabels 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-N-oxyl-4-acetylene (TEMPA) 6 and (15) N-labeled TEMPA 6* and their coupling to uridine. The yield of the synthesis of TEMPA could be increased to 40 % and the second nitroxide 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-(15) N-oxyl-4-acetylene 6* could be synthesized with a yield of 11 %.

Original language	English
Pages (from-to)	655-659
Number of pages	5
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	26
Issue number	6-7
DOIs	https://doi.org/10.1080/15257770701490522
Publication status	Published - 2007

Keywords

RNA structure
sonogashira cross-coupling
side-specific label
nitroxide
EPR
NITROXIDE
PELDOR

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10.1080/15257770701490522

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title = "The synthesis of EPR differentiable spinlabels and their coupling to uridine",

abstract = "For EPR measurements of RNA, DNA, or proteins, the occurrence of the paramagnetic species is necessary. The aim of this work is to improve the synthesis of two different EPR spinlabels 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-N-oxyl-4-acetylene (TEMPA) 6 and (15) N-labeled TEMPA 6* and their coupling to uridine. The yield of the synthesis of TEMPA could be increased to 40 % and the second nitroxide 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-(15) N-oxyl-4-acetylene 6* could be synthesized with a yield of 11 %.",

keywords = "RNA structure, sonogashira cross-coupling, side-specific label, nitroxide, EPR, NITROXIDE, PELDOR",

author = "Olga Frolow and Bode, {Bela E.} and Angels, {Joachim W.}",

year = "2007",

doi = "10.1080/15257770701490522",

language = "English",

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pages = "655--659",

journal = "Nucleosides, Nucleotides and Nucleic Acids",

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publisher = "Taylor and Francis",

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T1 - The synthesis of EPR differentiable spinlabels and their coupling to uridine

AU - Frolow, Olga

AU - Bode, Bela E.

AU - Angels, Joachim W.

PY - 2007

Y1 - 2007

N2 - For EPR measurements of RNA, DNA, or proteins, the occurrence of the paramagnetic species is necessary. The aim of this work is to improve the synthesis of two different EPR spinlabels 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-N-oxyl-4-acetylene (TEMPA) 6 and (15) N-labeled TEMPA 6* and their coupling to uridine. The yield of the synthesis of TEMPA could be increased to 40 % and the second nitroxide 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-(15) N-oxyl-4-acetylene 6* could be synthesized with a yield of 11 %.

AB - For EPR measurements of RNA, DNA, or proteins, the occurrence of the paramagnetic species is necessary. The aim of this work is to improve the synthesis of two different EPR spinlabels 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-N-oxyl-4-acetylene (TEMPA) 6 and (15) N-labeled TEMPA 6* and their coupling to uridine. The yield of the synthesis of TEMPA could be increased to 40 % and the second nitroxide 2,2,6,6-tetramethyl-3,4-dehydro-piperidin-(15) N-oxyl-4-acetylene 6* could be synthesized with a yield of 11 %.

KW - RNA structure

KW - sonogashira cross-coupling

KW - side-specific label

KW - nitroxide

KW - EPR

KW - NITROXIDE

KW - PELDOR

U2 - 10.1080/15257770701490522

DO - 10.1080/15257770701490522

M3 - Article

SN - 1525-7770

VL - 26

SP - 655

EP - 659

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 6-7

ER -

The synthesis of EPR differentiable spinlabels and their coupling to uridine

Abstract

Keywords

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