The shelterin protein POT-1 anchors Caenorhabditis elegans telomeres through SUN-1 at the nuclear periphery

Helder C. Ferreira, Benjamin D. Towbin, Thibaud Jegou, Susan M. Gasser*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Telomeres are specialized protein DNA structures that protect chromosome ends. In budding yeast, telomeres form clusters at the nuclear periphery. By imaging telomeres in embryos of the metazoan Caenorhabditis elegans, we found that telomeres clustered only in strains that had activated an alternative telomere maintenance pathway (ALT). Moreover, as in yeast, the unclustered telomeres in wild-type embryos were located near the nuclear envelope (NE). This bias for perinuclear localization increased during embryogenesis and persisted in differentiated cells. Telomere position in early embryos required the NE protein SUN-1, the single-strand binding protein POT-1, and the small ubiquitin-like modifier (SUMO) ligase GEI-17. However, in postmitotic larval cells, none of these factors individually were required for telomere anchoring, which suggests that additional mechanisms anchor in late development. Importantly, targeted POT-1 was sufficient to anchor chromatin to the NE in a SUN-1 dependent manner, arguing that its effect at telomeres is direct. This high-resolution description of telomere position within C. elegans extends our understanding of telomere organization in eukaryotes.

Original languageEnglish
Pages (from-to)727-735
Number of pages9
JournalJournal of Cell Biology
Volume203
Issue number5
DOIs
Publication statusPublished - 9 Dec 2013

Keywords

  • C-ELEGANS
  • YEAST TELOMERES
  • SIR4 PROTEINS
  • BUDDING YEAST
  • PML BODY
  • ORGANIZATION
  • ENVELOPE
  • DNA
  • RECOMBINATION
  • CHROMOSOMES

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