The rapid isolation and growth dynamics of the tsetse symbiont Sodalis glossinidius

C Z Matthew, A C Darby, Simon Young, L H Hume, S C Welburn

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Sodalis glossinidius is known exclusively in endosymbiosis with tsetse flies (Genus: Glossina) and is one of the few insect bacterial symbionts that have been successfully cultured in vitro. This study details improved isolation and solid culture protocols that allow for a standardised and rapid preparation/maintenance of clonal material from individual flies. The isolation and culture of S. glossinidius was confirmed by partial sequencing of the 16S rDNA gene and specific PCR. In addition, the growth dynamics and changes in cell viability during liquid culture are described. The potential for culture of other endosymbiont taxa, is discussed. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)69-74
Number of pages6
JournalFEMS Microbiology Letters
Volume248
Issue number1
DOIs
Publication statusPublished - 1 Jul 2005

Keywords

  • Sodalis glossinidius
  • culture
  • insect symbiont
  • tsetse fly
  • microacrophilic
  • RICKETTSIA-LIKE-ORGANISMS
  • SECONDARY SYMBIONTS
  • FLIES GLOSSINA
  • MORSITANS
  • BACTERIA
  • INSECTS
  • TISSUES
  • MIDGUT

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