The rapid isolation and growth dynamics of the tsetse symbiont Sodalis glossinidius

C Z  Matthew; A C  Darby; Simon Young; L H  Hume; S C  Welburn

doi:10.1016/j.femsle.2005.05.024

The rapid isolation and growth dynamics of the tsetse symbiont Sodalis glossinidius

C Z Matthew, A C Darby, Simon Young, L H Hume, S C Welburn

Research output: Contribution to journal › Article › peer-review

Abstract

Sodalis glossinidius is known exclusively in endosymbiosis with tsetse flies (Genus: Glossina) and is one of the few insect bacterial symbionts that have been successfully cultured in vitro. This study details improved isolation and solid culture protocols that allow for a standardised and rapid preparation/maintenance of clonal material from individual flies. The isolation and culture of S. glossinidius was confirmed by partial sequencing of the 16S rDNA gene and specific PCR. In addition, the growth dynamics and changes in cell viability during liquid culture are described. The potential for culture of other endosymbiont taxa, is discussed. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	69-74
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	248
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2005.05.024
Publication status	Published - 1 Jul 2005

Keywords

Sodalis glossinidius
culture
insect symbiont
tsetse fly
microacrophilic
RICKETTSIA-LIKE-ORGANISMS
SECONDARY SYMBIONTS
FLIES GLOSSINA
MORSITANS
BACTERIA
INSECTS
TISSUES
MIDGUT

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10.1016/j.femsle.2005.05.024

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title = "The rapid isolation and growth dynamics of the tsetse symbiont Sodalis glossinidius",

abstract = "Sodalis glossinidius is known exclusively in endosymbiosis with tsetse flies (Genus: Glossina) and is one of the few insect bacterial symbionts that have been successfully cultured in vitro. This study details improved isolation and solid culture protocols that allow for a standardised and rapid preparation/maintenance of clonal material from individual flies. The isolation and culture of S. glossinidius was confirmed by partial sequencing of the 16S rDNA gene and specific PCR. In addition, the growth dynamics and changes in cell viability during liquid culture are described. The potential for culture of other endosymbiont taxa, is discussed. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.",

keywords = "Sodalis glossinidius, culture, insect symbiont, tsetse fly, microacrophilic, RICKETTSIA-LIKE-ORGANISMS, SECONDARY SYMBIONTS, FLIES GLOSSINA, MORSITANS, BACTERIA, INSECTS, TISSUES, MIDGUT",

author = "Matthew, {C Z} and Darby, {A C} and Simon Young and Hume, {L H} and Welburn, {S C}",

year = "2005",

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language = "English",

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AU - Matthew, C Z

AU - Darby, A C

AU - Young, Simon

AU - Hume, L H

AU - Welburn, S C

PY - 2005/7/1

Y1 - 2005/7/1

N2 - Sodalis glossinidius is known exclusively in endosymbiosis with tsetse flies (Genus: Glossina) and is one of the few insect bacterial symbionts that have been successfully cultured in vitro. This study details improved isolation and solid culture protocols that allow for a standardised and rapid preparation/maintenance of clonal material from individual flies. The isolation and culture of S. glossinidius was confirmed by partial sequencing of the 16S rDNA gene and specific PCR. In addition, the growth dynamics and changes in cell viability during liquid culture are described. The potential for culture of other endosymbiont taxa, is discussed. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

AB - Sodalis glossinidius is known exclusively in endosymbiosis with tsetse flies (Genus: Glossina) and is one of the few insect bacterial symbionts that have been successfully cultured in vitro. This study details improved isolation and solid culture protocols that allow for a standardised and rapid preparation/maintenance of clonal material from individual flies. The isolation and culture of S. glossinidius was confirmed by partial sequencing of the 16S rDNA gene and specific PCR. In addition, the growth dynamics and changes in cell viability during liquid culture are described. The potential for culture of other endosymbiont taxa, is discussed. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

KW - Sodalis glossinidius

KW - culture

KW - insect symbiont

KW - tsetse fly

KW - microacrophilic

KW - RICKETTSIA-LIKE-ORGANISMS

KW - SECONDARY SYMBIONTS

KW - FLIES GLOSSINA

KW - MORSITANS

KW - BACTERIA

KW - INSECTS

KW - TISSUES

KW - MIDGUT

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DO - 10.1016/j.femsle.2005.05.024

M3 - Article

SN - 0378-1097

VL - 248

SP - 69

EP - 74

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

The rapid isolation and growth dynamics of the tsetse symbiont Sodalis glossinidius

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Keywords

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