The presence of angiotensin II receptors in elasmobranchs

The presence of specific Ang II receptors in membrane fractions was investigated using I-125-labeled homologous Ang II ([Asn(1), Pro(3), Ile(5)]Ang II; df Ang II) in Triakis scyllia. Specific binding sites occurred in a variety of tissues, with highest binding in interrenal tissue (17.11 +/- 2.45 fmol Ang II/mg protein) and gill (6.26 +/- 0.69 fmol Ang II/mg protein) and possible Ang II receptors in rectal gland and other tissues. I-125-[Asn(1), Pro(3), Ile(5)]Ang II (10(-10)M) binding to branchial cell membrane fraction (25 mu g protein) in 5 mM MgCl2, 125 mM NaCl, 50 mM Tris-HCl, 0.2% bovine serum albumin at 28 degrees (1) is rapid and saturable; (2) increases as a function of membrane concentration and time; and (3) optimally fits to a two-site (high- and low-affinity) model. The equilibrium dissociation constant (0.11 +/- 0.01 nM) and binding site concentration (35.00 +/- 1.16 fmol/mg protein) are similar to those of mammalian and avian vascular Ang II receptors. Bound labeled ligand was not competitively displaced by dogfish Ang I, dogfish C-type natriuretic peptide, bradykinin, or the AT(1) receptor antagonist, CV 11974. The AT(2) receptor antagonist, CGP 42112, was much less potent at displacing the labeled ligand compared to the unlabeled ligand. (C) Academic Press