The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast

Helder Casimiro Ferreira, Brian Luke, Heiko Schober, Veronique Kalck, Joachim Lingner, Susan M. Gasser*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

77 Citations (Scopus)

Abstract

Budding yeast telomeres are reversibly bound at the nuclear envelope through two partially redundant pathways that involve the Sir2/3/4 silencing complex and the Yku70/80 heterodimer(1,2). To better understand how this is regulated, we studied the role of SUMOylation in telomere anchoring. We find that the PIAS-like SUMO E3 ligase Siz2 sumoylates both Yku70/80 and Sir4 in vivo and promotes telomere anchoring to the nuclear envelope. Remarkably, loss of Siz2 also provokes telomere extension in a telomerase-dependent manner that is epistatic with loss of the helicase Pif1. Consistent with our previously documented role for telomerase in anchorage(3), normal telomere anchoring in siz2 Delta is restored by PIF1 deletion. By live-cell imaging of a critically short telomere, we show that telomeres shift a way from the nuclear envelope when elongating. We propose that SUMO-dependent association with the nuclear periphery restrains bound telomerase, whereas active elongation correlates with telomere release.

Original languageEnglish
Pages (from-to)867-874
JournalNature Cell Biology
Volume13
Issue number7
DOIs
Publication statusPublished - Jul 2011

Keywords

  • SUMOYLATION
  • ENZYME
  • DOMAIN PROTEIN MPS3
  • NUCLEAR-PORE COMPLEX
  • DNA-REPAIR
  • S-PHASE
  • SUMO MODIFICATION
  • SACCHAROMYCES-CEREVISIAE
  • LENGTH
  • KU

Fingerprint

Dive into the research topics of 'The PIAS homologue Siz2 regulates perinuclear telomere position and telomerase activity in budding yeast'. Together they form a unique fingerprint.

Cite this