TY - JOUR
T1 - The New World arenavirus Tacaribe virus induces caspase-dependent apoptosis in infected cells
AU - Wolff, Svenja
AU - Groseth, Allison
AU - Meyer, Bjoern
AU - Jackson, David
AU - Strecker, Thomas
AU - Kaufmann, Andreas
AU - Becker, Stephan
N1 - This work was supported in part by fellowships from the Jürgen Manchot
Stiftung (S.W.; http://www.manchot.org), the German Research Council [SFB 1021 TP A04 (A.K.), SFB 1021 TP B03 (S.B., S.W.) and SFB 1021 TP B05 (T.S.); http://www.uni-marburg.de/sfb1021], the Canadian Institutes of Health Research (A.G.; http://www.cihr-irsc.gc.ca) and a Medical Research Council Centenary Travel Award (B.M.).
PY - 2016/4
Y1 - 2016/4
N2 - The Arenaviridae is a diverse and growing family of viruses that already includes more than 25 distinct species. While some of these viruses have a significant impact on public health, others appear to be non-pathogenic. At present little is known about the host cell responses to infection with different arenaviruses, particularly those found in the New World; however, apoptosis is known to play an important role in controlling infection of many viruses. Here we show that infection with Tacaribe virus (TCRV), which is widely considered the prototype for non-pathogenic arenaviruses, leads to stronger induction of apoptosis than does infection with its human-pathogenic relative Junín virus. TCRV-induced apoptosis occurred in several cell types during late stages of infection and was shown to be caspase-dependent, involving the activation of caspases 3, 7, 8 and 9. Further, UV-inactivated TCRV did not induce apoptosis, indicating that the activation of this process is dependent on active viral replication/transcription. Interestingly, when apoptosis was inhibited, growth of TCRV was not enhanced, indicating that apoptosis does not have a direct negative effect on TCRV infection in vitro. Taken together, our data identify and characterize an important virus-host cell interaction of the prototypic, non-pathogenic arenavirus TCRV, which provides important insight into the growing field of arenavirus research aimed at better understanding the diversity in responses to different arenavirus infections and their functional consequences.
AB - The Arenaviridae is a diverse and growing family of viruses that already includes more than 25 distinct species. While some of these viruses have a significant impact on public health, others appear to be non-pathogenic. At present little is known about the host cell responses to infection with different arenaviruses, particularly those found in the New World; however, apoptosis is known to play an important role in controlling infection of many viruses. Here we show that infection with Tacaribe virus (TCRV), which is widely considered the prototype for non-pathogenic arenaviruses, leads to stronger induction of apoptosis than does infection with its human-pathogenic relative Junín virus. TCRV-induced apoptosis occurred in several cell types during late stages of infection and was shown to be caspase-dependent, involving the activation of caspases 3, 7, 8 and 9. Further, UV-inactivated TCRV did not induce apoptosis, indicating that the activation of this process is dependent on active viral replication/transcription. Interestingly, when apoptosis was inhibited, growth of TCRV was not enhanced, indicating that apoptosis does not have a direct negative effect on TCRV infection in vitro. Taken together, our data identify and characterize an important virus-host cell interaction of the prototypic, non-pathogenic arenavirus TCRV, which provides important insight into the growing field of arenavirus research aimed at better understanding the diversity in responses to different arenavirus infections and their functional consequences.
U2 - 10.1099/jgv.0.000403
DO - 10.1099/jgv.0.000403
M3 - Article
C2 - 26769540
SN - 0022-1317
VL - 97
SP - 855
EP - 866
JO - Journal of General Virology
JF - Journal of General Virology
IS - 4
ER -