The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome

Mohammed Sebaihia; Brendan W. Wren; Peter Mullany; Neil F. Fairweather; Nigel Minton; Richard Stabler; Nicholas R. Thomson; Adam P. Roberts; Ana M. Cerdeno-Tarrraga; Hongmei Wang; Matthew T. G. Holden; Anne Wright; Carol Churcher; Michael A. Quail; Stephen Baker; Nathalie Bason; Karen Brooks; Tracey Chillingworth; Ann Cronin; Paul Davis; Linda Dowd; Audrey Fraser; Theresa Feltwell; Zahra Hance; Simon Holroyd; Kay Jagels; Sharon Moule; Karen Mungall; Claire Price; Ester Rabbinowitsch; Sarah Sharp; Mark Simmonds; Kim Stevens; Louise Unwin; Sally Whithead; Bruno Dupuy; Gordon Dougan; Bart Barrell; Julian Parkhill

doi:10.1038/ng1830

The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome

Mohammed Sebaihia, Brendan W. Wren, Peter Mullany, Neil F. Fairweather, Nigel Minton, Richard Stabler, Nicholas R. Thomson, Adam P. Roberts, Ana M. Cerdeno-Tarrraga, Hongmei Wang, Matthew T. G. Holden, Anne Wright, Carol Churcher, Michael A. Quail, Stephen Baker, Nathalie Bason, Karen Brooks, Tracey Chillingworth, Ann Cronin, Paul DavisLinda Dowd, Audrey Fraser, Theresa Feltwell, Zahra Hance, Simon Holroyd, Kay Jagels, Sharon Moule, Karen Mungall, Claire Price, Ester Rabbinowitsch, Sarah Sharp, Mark Simmonds, Kim Stevens, Louise Unwin, Sally Whithead, Bruno Dupuy, Gordon Dougan, Bart Barrell, Julian Parkhill^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.

Original language	English
Pages (from-to)	779-786
Number of pages	8
Journal	Nature Genetics
Volume	38
Issue number	7
DOIs	https://doi.org/10.1038/ng1830
Publication status	Published - Jul 2006

Keywords

GRAM-POSITIVE BACTERIA
SPORE GERMINATION
STAPHYLOCOCCUS-AUREUS
ENTEROCOCCUS-FAECALIS
CONJUGAL TRANSFER
SURFACE PROTEIN
GENE
VIRULENCE
SEQUENCE
PERFRINGENS

Access to Document

10.1038/ng1830

Cite this

Sebaihia, M., Wren, B. W., Mullany, P., Fairweather, N. F., Minton, N., Stabler, R., Thomson, N. R., Roberts, A. P., Cerdeno-Tarrraga, A. M., Wang, H., Holden, M. T. G., Wright, A., Churcher, C., Quail, M. A., Baker, S., Bason, N., Brooks, K., Chillingworth, T., Cronin, A., ... Parkhill, J. (2006). The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome. Nature Genetics, 38(7), 779-786. https://doi.org/10.1038/ng1830

@article{b446138211094dc9a17597688af4b110,

title = "The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome",

abstract = "We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.",

keywords = "GRAM-POSITIVE BACTERIA, SPORE GERMINATION, STAPHYLOCOCCUS-AUREUS, ENTEROCOCCUS-FAECALIS, CONJUGAL TRANSFER, SURFACE PROTEIN, GENE, VIRULENCE, SEQUENCE, PERFRINGENS",

author = "Mohammed Sebaihia and Wren, {Brendan W.} and Peter Mullany and Fairweather, {Neil F.} and Nigel Minton and Richard Stabler and Thomson, {Nicholas R.} and Roberts, {Adam P.} and Cerdeno-Tarrraga, {Ana M.} and Hongmei Wang and Holden, {Matthew T. G.} and Anne Wright and Carol Churcher and Quail, {Michael A.} and Stephen Baker and Nathalie Bason and Karen Brooks and Tracey Chillingworth and Ann Cronin and Paul Davis and Linda Dowd and Audrey Fraser and Theresa Feltwell and Zahra Hance and Simon Holroyd and Kay Jagels and Sharon Moule and Karen Mungall and Claire Price and Ester Rabbinowitsch and Sarah Sharp and Mark Simmonds and Kim Stevens and Louise Unwin and Sally Whithead and Bruno Dupuy and Gordon Dougan and Bart Barrell and Julian Parkhill",

year = "2006",

month = jul,

doi = "10.1038/ng1830",

language = "English",

volume = "38",

pages = "779--786",

journal = "Nature Genetics",

issn = "1061-4036",

publisher = "Nature publishing group",

number = "7",

}

Sebaihia, M, Wren, BW, Mullany, P, Fairweather, NF, Minton, N, Stabler, R, Thomson, NR, Roberts, AP, Cerdeno-Tarrraga, AM, Wang, H, Holden, MTG, Wright, A, Churcher, C, Quail, MA, Baker, S, Bason, N, Brooks, K, Chillingworth, T, Cronin, A, Davis, P, Dowd, L, Fraser, A, Feltwell, T, Hance, Z, Holroyd, S, Jagels, K, Moule, S, Mungall, K, Price, C, Rabbinowitsch, E, Sharp, S, Simmonds, M, Stevens, K, Unwin, L, Whithead, S, Dupuy, B, Dougan, G, Barrell, B & Parkhill, J 2006, 'The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome', Nature Genetics, vol. 38, no. 7, pp. 779-786. https://doi.org/10.1038/ng1830

TY - JOUR

T1 - The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome

AU - Sebaihia, Mohammed

AU - Wren, Brendan W.

AU - Mullany, Peter

AU - Fairweather, Neil F.

AU - Minton, Nigel

AU - Stabler, Richard

AU - Thomson, Nicholas R.

AU - Roberts, Adam P.

AU - Cerdeno-Tarrraga, Ana M.

AU - Wang, Hongmei

AU - Holden, Matthew T. G.

AU - Wright, Anne

AU - Churcher, Carol

AU - Quail, Michael A.

AU - Baker, Stephen

AU - Bason, Nathalie

AU - Brooks, Karen

AU - Chillingworth, Tracey

AU - Cronin, Ann

AU - Davis, Paul

AU - Dowd, Linda

AU - Fraser, Audrey

AU - Feltwell, Theresa

AU - Hance, Zahra

AU - Holroyd, Simon

AU - Jagels, Kay

AU - Moule, Sharon

AU - Mungall, Karen

AU - Price, Claire

AU - Rabbinowitsch, Ester

AU - Sharp, Sarah

AU - Simmonds, Mark

AU - Stevens, Kim

AU - Unwin, Louise

AU - Whithead, Sally

AU - Dupuy, Bruno

AU - Dougan, Gordon

AU - Barrell, Bart

AU - Parkhill, Julian

PY - 2006/7

Y1 - 2006/7

N2 - We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.

AB - We determined the complete genome sequence of Clostridium difficile strain 630, a virulent and multidrug-resistant strain. Our analysis indicates that a large proportion (11%) of the genome consists of mobile genetic elements, mainly in the form of conjugative transposons. These mobile elements are putatively responsible for the acquisition by C. difficile of an extensive array of genes involved in antimicrobial resistance, virulence, host interaction and the production of surface structures. The metabolic capabilities encoded in the genome show multiple adaptations for survival and growth within the gut environment. The extreme genome variability was confirmed by whole-genome microarray analysis; it may reflect the organism's niche in the gut and should provide information on the evolution of virulence in this organism.

KW - GRAM-POSITIVE BACTERIA

KW - SPORE GERMINATION

KW - STAPHYLOCOCCUS-AUREUS

KW - ENTEROCOCCUS-FAECALIS

KW - CONJUGAL TRANSFER

KW - SURFACE PROTEIN

KW - GENE

KW - VIRULENCE

KW - SEQUENCE

KW - PERFRINGENS

U2 - 10.1038/ng1830

DO - 10.1038/ng1830

M3 - Article

SN - 1061-4036

VL - 38

SP - 779

EP - 786

JO - Nature Genetics

JF - Nature Genetics

IS - 7

ER -

The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome

Abstract

Keywords

Access to Document

Fingerprint

Cite this