The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay

Andrea Rachow; Elmar Saathoff; Bariki Mtafya; Daniel Mapamba; Chacha Mangu; Gabriel Rojas-Ponce; Nyanda E. Ntinginya; Martin Boeree; Norbert Heinrich; Stephen H. Gillespie; Michael Hoelscher; PanACEA Consortium

doi:10.1016/j.tube.2018.04.001

The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay

Andrea Rachow, Elmar Saathoff, Bariki Mtafya, Daniel Mapamba, Chacha Mangu, Gabriel Rojas-Ponce, Nyanda E. Ntinginya, Martin Boeree, Norbert Heinrich, Stephen H. Gillespie, Michael Hoelscher, PanACEA Consortium

Research output: Contribution to journal › Article › peer-review

Abstract

The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (C_Tmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The C_Tmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that C_Tmin readouts of decontaminated sputum samples should be interpreted with caution.

Original language	English
Journal	Tuberculosis
Volume	In press
Early online date	4 Apr 2018
DOIs	https://doi.org/10.1016/j.tube.2018.04.001
Publication status	E-pub ahead of print - 4 Apr 2018

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Xpert-inhibition
Sputum processing
Quantitative PCR
Xpert-performance

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10.1016/j.tube.2018.04.001

Rachow_2018_Tuberculosis_XpertMTBRIF_AAM
© 2018 Elsevier Ltd. This work has been made available online in accordance with the publisher’s policies. This is the author created, accepted version manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1016/j.tube.2018.04.001
Accepted author manuscript, 667 KBLicence: CC BY-NC-ND

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Rachow, A., Saathoff, E., Mtafya, B., Mapamba, D., Mangu, C., Rojas-Ponce, G., Ntinginya, N. E., Boeree, M., Heinrich, N., Gillespie, S. H., Hoelscher, M., & PanACEA Consortium (2018). The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay. Tuberculosis, In press. Advance online publication. https://doi.org/10.1016/j.tube.2018.04.001

@article{76d5bd005b24490a8877669e6a9f5431,

title = "The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay",

abstract = "The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that CTmin readouts of decontaminated sputum samples should be interpreted with caution.",

keywords = "Xpert-inhibition, Sputum processing, Quantitative PCR, Xpert-performance",

author = "Andrea Rachow and Elmar Saathoff and Bariki Mtafya and Daniel Mapamba and Chacha Mangu and Gabriel Rojas-Ponce and Ntinginya, \{Nyanda E.\} and Martin Boeree and Norbert Heinrich and Gillespie, \{Stephen H.\} and Michael Hoelscher and \{PanACEA Consortium\}",

note = "The study was supported by the German Ministry of Education and Research (BMBF, Grant No. 01KA0901 ) and the European and Developing Countries Clinical Trials Partnership (EDCTP, Project-ID: IP.2007.32011.011 ). ",

year = "2018",

month = apr,

day = "4",

doi = "10.1016/j.tube.2018.04.001",

language = "English",

volume = "In press",

journal = "Tuberculosis",

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T1 - The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay

AU - Rachow, Andrea

AU - Saathoff, Elmar

AU - Mtafya, Bariki

AU - Mapamba, Daniel

AU - Mangu, Chacha

AU - Rojas-Ponce, Gabriel

AU - Ntinginya, Nyanda E.

AU - Boeree, Martin

AU - Heinrich, Norbert

AU - Gillespie, Stephen H.

AU - Hoelscher, Michael

AU - PanACEA Consortium

N1 - The study was supported by the German Ministry of Education and Research (BMBF, Grant No. 01KA0901 ) and the European and Developing Countries Clinical Trials Partnership (EDCTP, Project-ID: IP.2007.32011.011 ).

PY - 2018/4/4

Y1 - 2018/4/4

N2 - The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that CTmin readouts of decontaminated sputum samples should be interpreted with caution.

AB - The Xpert MTB/RIF assay detects Mycobacterium tuberculosis in unprocessed or NALC/NaOH- decontaminated sputum. The effect of repeated NALC/NaOH-decontamination on several Xpert performance parameters was assessed in this study. A second NALC/NaOH-decontamination had no effect on the binary Xpert-outcome but increased the value for the quantitative readout (CTmin). Repeated decontamination was not associated with PCR-inhibition or invalid results. The CTmin of M.tb positive samples was higher in inhibited Xpert runs. Our data indicate that NALC/NaOH-decontamination has an effect on the performance of the Xpert assay, and that CTmin readouts of decontaminated sputum samples should be interpreted with caution.

KW - Xpert-inhibition

KW - Sputum processing

KW - Quantitative PCR

KW - Xpert-performance

UR - https://www.scopus.com/pages/publications/85055635262

U2 - 10.1016/j.tube.2018.04.001

DO - 10.1016/j.tube.2018.04.001

M3 - Article

SN - 1472-9792

VL - In press

JO - Tuberculosis

JF - Tuberculosis

ER -

The impact of repeated NALC/NaOH- decontamination on the performance of Xpert MTB/RIF assay

Abstract

UN SDGs

Keywords

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