The hydrophobic region of the Leishmania peroxin 14: requirements for association with a glycosome mimetic membrane

Normand Cyr, Terry K. Smith, Élodie Boisselier, Louis-Philippe Leroux, Anwar Hasil Kottarampatel, Amanda Davidsen, Christian Salesse, Armando Jardim*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
2 Downloads (Pure)

Abstract

Protein import into the Leishmania glycosome requires docking of the cargo-loaded peroxin 5 (PEX5) receptor to the peroxin 14 (PEX14) bound to the glycosome surface. To examine the LdPEX14-membrane interaction, we purified L. donovani promastigote glycosomes and determined the phospholipid and fatty acid composition. These membranes contained predominately phosphatidylethanolamine, phosphatidylcholine, and phosphatidylglycerol (PG) modified primarily with C18 and C22 unsaturated fatty acid. Using large unilamellar vesicles (LUVs) with a lipid composition mimicking the glycosomal membrane in combination with sucrose density centrifugation and fluorescence-activated cell sorting technique, we established that the LdPEX14 membrane-binding activity was dependent on a predicted transmembrane helix found within residues 149-179. Monolayer experiments showed that the incorporation of PG and phospholipids with unsaturated fatty acids, which increase membrane fluidity and favor a liquid expanded phase, facilitated the penetration of LdPEX14 into biological membranes. Moreover, we demonstrated that the binding of LdPEX5 receptor or LdPEX5-PTS1 receptor-cargo complex was contingent on the presence of LdPEX14 at the surface of LUVs.

Original languageEnglish
Pages (from-to)511-529
Number of pages19
JournalBiochemical Journal
Volume475
Issue number2
DOIs
Publication statusPublished - 31 Jan 2018

Keywords

  • Leishmania
  • PEX14
  • Glycosome
  • Membrane proteins
  • Peroxin
  • Transmembrane proteins

Fingerprint

Dive into the research topics of 'The hydrophobic region of the Leishmania peroxin 14: requirements for association with a glycosome mimetic membrane'. Together they form a unique fingerprint.

Cite this