The heme pocket of the globin domain of the globin-coupled sensor of Geobacter sulfurreducens - An EPR study

Filip Desmet; Liesbet Thijs; Hassane El Mkami; Sylvia Dewilde; Luc Moens; Graham Murray Smith; Sabine Van Doorslaer

doi:10.1016/j.jinorgbio.2010.05.009

The heme pocket of the globin domain of the globin-coupled sensor of Geobacter sulfurreducens - An EPR study

Filip Desmet, Liesbet Thijs, Hassane El Mkami, Sylvia Dewilde, Luc Moens, Graham Murray Smith, Sabine Van Doorslaer

School of Physics and Astronomy

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

The globin-coupled sensor (GCS) of Geobacter sulfurreducens is unique amongst GCSs in that its signalling domain is a transmembrane domain with yet unknown function. In the present work we use X-band continuous-wave and pulsed electron paramagnetic resonance (EPR) to investigate the ferric form of the globin domain of the G. sulfurreducens GCS (GsGCS(162)) at pH 8.5. This form shows a unique bis-histidine coordination of the heme with the F8His and E11His. In contrast with previous crystal structure data, where three conformers of the heme structure were identified, ferric GsGCS(162) assumes only one conformation in frozen solution. The EPR data of ferric GsGCS(162) are compared in detail with those of other bis-histidine coordinated globins, including other GCS systems. (c) 2010 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	1022-1028
Number of pages	7
Journal	Journal of Inorganic Biochemistry
Volume	104
Issue number	10
DOIs	https://doi.org/10.1016/j.jinorgbio.2010.05.009
Publication status	Published - Oct 2010

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10.1016/j.jinorgbio.2010.05.009

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title = "The heme pocket of the globin domain of the globin-coupled sensor of Geobacter sulfurreducens - An EPR study",

abstract = "The globin-coupled sensor (GCS) of Geobacter sulfurreducens is unique amongst GCSs in that its signalling domain is a transmembrane domain with yet unknown function. In the present work we use X-band continuous-wave and pulsed electron paramagnetic resonance (EPR) to investigate the ferric form of the globin domain of the G. sulfurreducens GCS (GsGCS(162)) at pH 8.5. This form shows a unique bis-histidine coordination of the heme with the F8His and E11His. In contrast with previous crystal structure data, where three conformers of the heme structure were identified, ferric GsGCS(162) assumes only one conformation in frozen solution. The EPR data of ferric GsGCS(162) are compared in detail with those of other bis-histidine coordinated globins, including other GCS systems. (c) 2010 Elsevier Inc. All rights reserved.",

author = "Filip Desmet and Liesbet Thijs and {El Mkami}, Hassane and Sylvia Dewilde and Luc Moens and Smith, {Graham Murray} and {Van Doorslaer}, Sabine",

year = "2010",

month = oct,

doi = "10.1016/j.jinorgbio.2010.05.009",

language = "English",

volume = "104",

pages = "1022--1028",

journal = "Journal of Inorganic Biochemistry",

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T1 - The heme pocket of the globin domain of the globin-coupled sensor of Geobacter sulfurreducens - An EPR study

AU - Desmet, Filip

AU - Thijs, Liesbet

AU - El Mkami, Hassane

AU - Dewilde, Sylvia

AU - Moens, Luc

AU - Smith, Graham Murray

AU - Van Doorslaer, Sabine

PY - 2010/10

Y1 - 2010/10

N2 - The globin-coupled sensor (GCS) of Geobacter sulfurreducens is unique amongst GCSs in that its signalling domain is a transmembrane domain with yet unknown function. In the present work we use X-band continuous-wave and pulsed electron paramagnetic resonance (EPR) to investigate the ferric form of the globin domain of the G. sulfurreducens GCS (GsGCS(162)) at pH 8.5. This form shows a unique bis-histidine coordination of the heme with the F8His and E11His. In contrast with previous crystal structure data, where three conformers of the heme structure were identified, ferric GsGCS(162) assumes only one conformation in frozen solution. The EPR data of ferric GsGCS(162) are compared in detail with those of other bis-histidine coordinated globins, including other GCS systems. (c) 2010 Elsevier Inc. All rights reserved.

AB - The globin-coupled sensor (GCS) of Geobacter sulfurreducens is unique amongst GCSs in that its signalling domain is a transmembrane domain with yet unknown function. In the present work we use X-band continuous-wave and pulsed electron paramagnetic resonance (EPR) to investigate the ferric form of the globin domain of the G. sulfurreducens GCS (GsGCS(162)) at pH 8.5. This form shows a unique bis-histidine coordination of the heme with the F8His and E11His. In contrast with previous crystal structure data, where three conformers of the heme structure were identified, ferric GsGCS(162) assumes only one conformation in frozen solution. The EPR data of ferric GsGCS(162) are compared in detail with those of other bis-histidine coordinated globins, including other GCS systems. (c) 2010 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.jinorgbio.2010.05.009

DO - 10.1016/j.jinorgbio.2010.05.009

M3 - Article

SN - 0162-0134

VL - 104

SP - 1022

EP - 1028

JO - Journal of Inorganic Biochemistry

JF - Journal of Inorganic Biochemistry

IS - 10

ER -

The heme pocket of the globin domain of the globin-coupled sensor of Geobacter sulfurreducens - An EPR study

Abstract

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