Abstract
Results. We generate a high quality genome assembly for G. rostochiensis pathotype Ro1, identify putative effectors and horizontal gene transfer events, map gene expression through the life cycle focusing on key parasitic transitions and sequence the genomes of eight populations including four additional pathotypes to identify variation. Horizontal gene transfer contributes 3.5 % of the predicted genes, of which approximately 8.5 % are deployed as effectors. Over one-third of all effector genes are clustered in 21 putative ‘effector islands’ in the genome. We identify a dorsal gland promoter element motif (termed DOG Box) present upstream in representatives from 26 out of 28 dorsal gland effector families, and predict a putative effector superset associated with this motif. We validate gland cell expression in two novel genes by in situ hybridisation and catalogue dorsal gland promoter element-containing effectors from available cyst nematode genomes. Comparison of effector diversity between pathotypes highlights correlation with plant resistance-breaking.
Conclusions. These G. rostochiensis genome resources will facilitate major advances in understanding nematode plant-parasitism. Dorsal gland promoter element-containing effectors are at the front line of the evolutionary arms race between plant and parasite and the ability to predict gland cell expression a priori promises rapid advances in understanding their roles and mechanisms of action.
Original language | English |
---|---|
Article number | 124 |
Number of pages | 23 |
Journal | Genome Biology |
Volume | 17 |
DOIs | |
Publication status | Published - 10 Jun 2016 |
Keywords
- Plant-parasitic nematode
- Genome sequence
- Virulence
- Effectors
- Horizontal gene transfer
Fingerprint
Dive into the research topics of 'The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence'. Together they form a unique fingerprint.Profiles
Datasets
-
Data from: The genome of the yellow potato cyst nematode Globodera rostochiensis reveals insights into the basis of parasitism and virulence
Eves-van den Akker, S. (Creator), Laetsch, D. R. (Creator), Thorpe, P. J. (Creator), Lilley, C. J. (Creator), Danchin, E. G. J. (Creator), Da Rocha, M. (Creator), Rancurel, C. (Creator), Holroyd, N. E. (Creator), Cotton, J. A. (Creator), Szitenberg, A. (Creator), Grenier, E. (Creator), Montarry, J. (Creator), Mimee, B. (Creator), Duceppe, M.-O. (Creator), Boyes, I. (Creator), Marvin, J. M. C. (Creator), Jones, L. M. (Creator), Yusup, H. B. (Creator), Lafond-Lapalme, J. (Creator), Esquibet, M. (Creator), Sabeh, M. (Creator), Rott, M. (Creator), Overmars, H. (Creator), Finkers-Tomczak, A. (Creator), Smant, G. (Creator), Koutsovoulos, G. (Creator), Blok, V. (Creator), Mantelin, S. (Creator), Cock, P. J. A. (Creator), Phillips, W. (Creator), Henrissat, B. (Creator), Urwin, P. E. (Creator), Blaxter, M. (Creator) & Jones, J. (Creator), Dryad, 11 May 2016
DOI: 10.5061/dryad.4s5r6
Dataset
-
The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence (genomic dataset)
Eves-van den Akker, S. (Creator), Laetsch, D. R. (Creator), Thorpe, P. J. (Creator), Lilley, C. J. (Creator), Danchin, E. G. J. (Creator), Da Rocha, M. (Creator), Rancurel, C. (Creator), Holroyd, N. E. (Creator), Cotton, J. A. (Creator), Szitenberg, A. (Creator), Grenier, E. (Creator), Montarry, J. (Creator), Mimee, B. (Creator), Duceppe, M.-O. (Creator), Boyes, I. (Creator), Marvin, J. M. C. (Creator), Jones, L. M. (Creator), Yusup, H. B. (Creator), Lafond-Lapalme, J. (Creator), Esquibet, M. (Creator), Sabeh, M. (Creator), Rott, M. (Creator), Overmars, H. (Creator), Finkers-Tomczak, A. (Creator), Smant, G. (Creator), Koutsovoulos, G. (Creator), Blok, V. (Creator), Mantelin, S. (Creator), Cock, P. J. A. (Creator), Phillips, W. (Creator), Henrissat, B. (Creator), Urwin, P. E. (Creator), Blaxter, M. (Creator) & Jones, J. (Creator), National Center for Biotechnology Information, 2016
https://www.ncbi.nlm.nih.gov/bioproject/PRJEB12075/ and 3 more links, https://www.ncbi.nlm.nih.gov/sra/?term=ERR114519, https://www.ncbi.nlm.nih.gov/sra/?term=ERR123958, https://www.ncbi.nlm.nih.gov/sra/?term=ERR114520 (show fewer)
Dataset